Ribosomal scanning on the 5'-untranslated region of the human immunodeficiency virus RNA genome.
Ribosomal scanning on the 5'-untranslated region of the human immunodeficiency virus RNA genome.
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DOI：
10.1093/nar/gkr113
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发表时间：
2011-07
影响因子：
14.9
通讯作者：
Abbink TE
中科院分区：
文献类型：
--
作者：
Berkhout B;Arts K;Abbink TE
大多数真核 mRNA 的翻译起始是通过帽子依赖性扫描机制发生的，其效率由其 5'-非翻译区 (5'-UTR) 调节。人类免疫缺陷病毒 1 型 (HIV-1) 5'-UTR 在其 5' 末端直接含有一个稳定的 TAR 发夹，这可能掩盖了帽子结构。此外，5'-UTR相对较长，含有病毒复制所必需的几个稳定的RNA结构。这些特征可能会干扰核糖体扫描，并表明翻译是通过核糖体的内部进入启动的。关于 HIV-1 5'-UTR 驱动的翻译起始机制的文献存在争议。扫描和内部引发已被证明在各种实验系统中都发生。为了进一步了解翻译起始过程，我们确定了 5'-UTR 的哪一部分被扫描。为此，我们在 5'-UTR 的不同位置引入了上游 AUG，并确定了对置于 gag 起始密码子控制下的下游报告基因表达的影响。该策略使我们能够确定 HIV-1 5'-UTR 的核糖体扫描窗口。
Translation initiation on most eukaryotic mRNAs occurs via a cap-dependent scanning mechanism and its efficiency is modulated by their 5′-untranslated regions (5′-UTR). The human immunodeficiency virus type 1 (HIV-1) 5′-UTR contains a stable TAR hairpin directly at its 5′-end, which possibly masks the cap structure. In addition, the 5′-UTR is relatively long and contains several stable RNA structures that are essential for viral replication. These characteristics may interfere with ribosomal scanning and suggest that translation is initiated via internal entry of ribosomes. Literature on the HIV-1 5′-UTR-driven translation initiation mechanism is controversial. Both scanning and internal initiation have been shown to occur in various experimental systems. To gain further insight in the translation initiation process, we determined which part of the 5′-UTR is scanned. To do so, we introduced upstream AUGs at various positions across the 5′-UTR and determined the effect on expression of a downstream reporter gene that was placed under control of the gag start codon. This strategy allowed us to determine the window of ribosomal scanning on the HIV-1 5′-UTR.
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影响因子：
5.6
作者：
Damgaard, CK;Andersen, ES;Kjems, J
通讯作者：
Kjems, J
影响因子：
4.5
作者：
Babendure, JR;Babendure, JL;Tsien, RY
通讯作者：
Tsien, RY
DOI：
10.1073/pnas.0710650105
发表时间：
2008-03-25
影响因子：
11.1
作者：
Baranick, Brian T.;Lemp, Nathan A.;Logg, Christopher R.
通讯作者：
Logg, Christopher R.
影响因子：
5.4
作者：
Das, AT;Klaver, B;Berkhout, B
通讯作者：
Berkhout, B
影响因子：
64.5
作者：
BERKHOUT, B;SILVERMAN, RH;JEANG, KT
通讯作者：
JEANG, KT