Complete nucleotide sequence of a mumps virus genotype I strain isolated in Korea
Complete nucleotide sequence of a mumps virus genotype I strain isolated in Korea
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DOI：
10.1023/b:viru.0000016859.04203.88
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发表时间：
2004-03-01
期刊：
影响因子：
1.6
通讯作者：
Kang, C
中科院分区：
文献类型：
--
作者：
Lee, JY;Na, BK;Kang, C
测定了韩国分离的腮腺炎病毒Dg1062/Korea/98（Dg1062）的完整核苷酸序列。与其他腮腺炎病毒一样，其基因组长度为 15,384 个核苷酸 (nt)，编码 7 种蛋白质。通过RACE方法确认5'端和3'端分别为55和24nt。 Dg1062分离株的全长核苷酸序列在核苷酸序列水平上与其他菌株有2.9-6.8%的差异，导致相对于其他菌株的共有序列，仅在Dg1062分离株中观察到206个核苷酸和54个氨基酸取代。尽管包括 HN 基因在内的整个基因组的氨基酸存在变化，但可以认为该分离株的抗原位点没有显着变化。该结果是基因型I株全长基因组的首次报道，为流行性腮腺炎病毒遗传特征的多样性提供了概述。
The complete nucleotide sequence of mumps virus isolated in Korea, Dg1062/Korea/98 (Dg1062), was determined. As other mumps viruses, its genome was to be 15,384 nucleotides (nts) in length and encoded seven proteins. The both 5' and 3' ends were confirmed to be 55 and 24 nts by RACE method, respectively. The full-length nucleotide sequence of Dg1062 isolate differed from other strains by 2.9 - 6.8% in the nucleotide sequence level, resulting in 206 nucleotide and 54 amino acid substitutions which were observed in only Dg1062 isolate relative to the consensus sequences of other strains. Despite the variations of amino acids over the full genome including HN gene, it might be considered that this isolate have no significant variations in the antigenic sites. This result is the first report of full-length genome of genotype I strain and provides an overview on the diversity of genetic characteristics of circulating mumps virus.