Caveolin-1 isoforms are encoded by distinct mRNAs - Identification of mouse caveolin-1 mRNA variants caused by alternative transcription initiation and splicing
Caveolin-1 isoforms are encoded by distinct mRNAs - Identification of mouse caveolin-1 mRNA variants caused by alternative transcription initiation and splicing
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DOI：
10.1016/s0014-5793(99)01730-5
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发表时间：
2000-01-14
期刊：
影响因子：
3.5
通讯作者：
Fujimoto, T
中科院分区：
文献类型：
--
作者：
Kogo, H;Fujimoto, T
利用已知的编码α-caveolin-1的cDNA序列搜索EST数据库，（全长：FL），我们发现了一个变体，其第一个外显子的位置具有迄今未知的序列5 '-端变异体：5' V，5 'V mRNA表达水平与FL mRNA表达水平相当。FL和5 'V基因大小分别为2484 bp和2533 bp，除第一外显子外，其余序列完全相同。北方印迹分析表明，FL和5' V基因在肺、心脏和骨骼肌中均有强表达。以绿色荧光蛋白为标记物，对这些mRNA的蛋白质合成进行分析，发现FL mRNA主要产生α-异构体，而很少产生β-异构体。通过对小窝蛋白-1基因第一内含子的序列分析，发现在5 'V mRNA转录起始位点上游28 bp处存在一个TATA盒。这是第一次证明由交替转录起始产生的小窝蛋白-1 mRNA变体，它表明小窝蛋白-1的两种亚型是由两种不同的mRNA产生的。(C)2000年欧洲生物化学学会联合会。
By searching the EST database with the known cDNA sequence encoding alpha-caveolin-1 (full-length: FL), we found a variant having a hitherto unknown sequence in place of the first exon (5'-end variant: 5'V), The expression level of 5'V mRNA was equivalent to that of FL mRNA, The entire sequences of FL and 5'V mRNA were determined by 3'- and 5'-RACE analysis; their sizes were 2484 bp and 2533 bp, respectively, and the sequences were identical except for the region of the first exon, By Northern blotting, FL and 5'V mRNAs showed the same tissue distribution, and were intensely expressed in the lung, heart, and skeletal muscle. Analyzing the protein production from these mRNAs using green fluorescent protein as a tag, we found FL mRNA to produce the alpha-isoform predominantly, but to form little beta-isoform, The production of the beta-isoform from 5'V mRNA was also demonstrated. By sequence analysis of the first intron of the caveolin-1 gene, a TATA box was found at 28 bp upstream of the transcription initiation site for 5'V mRNA. This is the first demonstration of caveolin-1 mRNA variants generated by alternative transcription initiation, and it indicates that the two isoforms of caveolin-1 are produced from two distinct mRNAs. (C) 2000 Federation of European Biochemical Societies.