Crystallization and preliminary X-ray analysis of a monomeric mutant of Azami-Green (mAG), an Aequorea victoria green fluorescent protein-like green-emitting fluorescent protein from the stony coral Galaxea fascicularis.
Crystallization and preliminary X-ray analysis of a monomeric mutant of Azami-Green (mAG), an Aequorea victoria green fluorescent protein-like green-emitting fluorescent protein from the stony coral Galaxea fascicularis.
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Azami-Green (mAG) 单体突变体的结晶和初步 X 射线分析，Azami-Green 是一种来自石珊瑚 Galaxea fasciculis 的维多利亚水母绿色荧光蛋白样绿色发射荧光蛋白。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
M. Tanokura
中科院分区：
文献类型：
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作者：
Tatsuki Ebisawa;A. Yamamura;Y. Kameda;K. Hayakawa;K. Nagata;M. Tanokura
来自石珊瑚Galaxea fascicularis的单体Azami-绿色（mAG）是第一个不是维多利亚水母绿色荧光蛋白（avGFP）衍生物的单体绿色发射荧光蛋白。mAG和avGFP在氨基酸序列上有27%相同。使用PEG 3350作为沉淀剂，通过坐滴蒸气扩散法获得了衍射质量的重组mAG晶体。mAG晶体在Photon Factory（Tsukuba，Japan）的光束线AR-NW 12 A上衍射X射线至2.20 A分辨率。晶体属于空间群P1，晶胞参数a = 41.78，B = 51.72，c = 52.89 A，α = 90.96，β = 103.41，γ = 101.79度。马修斯系数（V（M）= 2.10 A（3）Da（-1））表明该晶体每个不对称单元含有两个mAG分子。
Monomeric Azami-Green (mAG) from the stony coral Galaxea fascicularis is the first monomeric green-emitting fluorescent protein that is not a derivative of Aequorea victoria green fluorescent protein (avGFP). mAG and avGFP are 27% identical in amino-acid sequence. Diffraction-quality crystals of recombinant mAG were obtained by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The mAG crystal diffracted X-rays to 2.20 A resolution on beamline AR-NW12A at the Photon Factory (Tsukuba, Japan). The crystal belonged to space group P1, with unit-cell parameters a = 41.78, b = 51.72, c = 52.89 A, alpha = 90.96, beta = 103.41, gamma = 101.79 degrees. The Matthews coefficient (V(M) = 2.10 A(3) Da(-1)) indicated that the crystal contained two mAG molecules per asymmetric unit.
影响因子：
2.9
作者：
Mishin, Alexander S.;Subach, Fedor V.;Verkhusha, Vladislav V.
通讯作者：
Verkhusha, Vladislav V.