Titanium Dioxide Nanoparticles Elicit Lower Direct Inhibitory Effect on Human Gut Microbiota Than Silver Nanoparticles
Titanium Dioxide Nanoparticles Elicit Lower Direct Inhibitory Effect on Human Gut Microbiota Than Silver Nanoparticles
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DOI：
10.1093/toxsci/kfz183
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发表时间：
2019-12-01
影响因子：
3.8
通讯作者：
Paliy, Oleg
中科院分区：
文献类型：
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作者：
Agans, Richard T.;Gordon, Alex;Paliy, Oleg
由于技术的不断发展，人们越来越多地接触现在用于食品和许多工业应用的工程纳米材料 (ENM)。历史上许多 ENM 已被证明具有抗菌特性，这引发了人们对膳食纳米材料如何通过微生物失调影响胃肠道健康的担忧。我们采用体外人体肠道模拟器系统来检查膳食纳米二氧化钛 (TiO2) 与人体肠道微生物群的相互作用。电子显微镜表明二氧化钛颗粒与细菌细胞密切相关。向微生物群落中添加 TiO2 会导致群落密度适度降低，但对群落多样性和均匀度没有影响。相比之下，在对照实验中施用已知的抗菌银纳米粒子（NP）会导致人口密度急剧下降。在这两种情况下，一旦停止添加纳米材料，社区就会恢复。群落概况的约束排序分析表明，模拟结肠区域是微生物群组成的主要决定因素。因此，微生物群暴露于 TiO2 后，预测的群落功能能力和测量的短链脂肪酸产量没有显着变化。我们得出的结论是，经过测试的 TiO2 纳米粒子对人类肠道微生物群的直接影响有限。
Due to continued technological development, people increasingly come in contact with engineered nanomaterials (ENMs) that are now used in foods and many industrial applications. Many ENMs have historically been shown to possess antimicrobial properties, which has sparked concern for how dietary nanomaterials impact gastrointestinal health via microbial dysbiosis. We employed an in vitro Human Gut Simulator system to examine interactions of dietary nano titanium dioxide (TiO2) with human gut microbiota. Electron microscopy indicated a close association of TiO2 particles with bacterial cells. Addition of TiO2 to microbial communities led to a modest reduction in community density but had no impact on community diversity and evenness. In contrast, administration of known antimicrobial silver nanoparticles (NPs) in a control experiment resulted in a drastic reduction of population density. In both cases, communities recovered once the addition of nanomaterials was ceased. Constrained ordination analysis of community profiles revealed that simulated colonic region was the primary determinant of microbiota composition. Accordingly, predicted community functional capacity and measured production of short-chain fatty acids were not changed significantly upon microbiota exposure to TiO2. We conclude that tested TiO2 NPs have limited direct effect on human gut microbiota.