EXCITATORY AND INHIBITORY AMINO-ACIDS AND PEPTIDE-INDUCED RESPONSES IN ACUTELY ISOLATED RAT SPINAL DORSAL HORN NEURONS
EXCITATORY AND INHIBITORY AMINO-ACIDS AND PEPTIDE-INDUCED RESPONSES IN ACUTELY ISOLATED RAT SPINAL DORSAL HORN NEURONS
复制标题
急性离体大鼠脊髓背角神经元的兴奋性和抑制性氨基酸及多肽诱导反应
DOI：
10.1016/0304-3940(89)90485-0
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发表时间：
1989-08-14
影响因子：
2.5
通讯作者：
RANDIC, M
中科院分区：
文献类型：
--
作者：
MURASE, K;RYU, PD;RANDIC, M
用全细胞电压钳技术研究了幼年大鼠脊髓背角神经元对兴奋性和抑制性氨基酸和肽的反应。用低浓度的酶和机械解离处理脊髓切片，产生分离的神经元，其对兴奋性氨基酸（谷氨酸、红藻氨酸、使君子氨酸和N-甲基-D-天冬氨酸（NMDA））、抑制性氨基酸（γ-天冬氨酸）敏感。氨基丁酸（GABA）、甘氨酸）和肽类（P物质、降钙素基因相关肽（CGRP））。背角神经元对NMDA的反应被甘氨酸和CGRP增强，而GABAA反应被α-氨基丁酸增强。氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）。我们的观察表明，有合理的协议之间的许多反应的分离神经元和那些在体内切片和培养制剂的研究。
The responses to excitatory and inhibitory amino acids and peptides were investigated in isolated rat spinal dorsal horn neurons (laminae I-V) of young rats using the whole-cell voltage-clamp technique. The treatment of spinal slices with low concentrations of enzymes and mechanical dissociation yielded isolated neurons that were sensitive to excitatory amino acids (glutamate, kainate, quisqualate and N-methyl-D-aspartate (NMDA), inhibitory amino acids (.gamma.-aminobutyric acid (GABA), glycine) and peptides (substance P, calcitonin gene-reached peptide (CGRP). The responses of dorsal horn neurons to NMDA were potentiated by glycine and CGRP, whereas GABAA responses were enhanced by .alpha.-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA). Our observation indicate that there is reasonable agreement between many of the responses of isolated neurons and those studied in in vivo slice and culture preparations.