Transgenerational conditioned male fertility of HD-ZIP IV transcription factor mutant ocl4: impact on 21-nt phasiRNA accumulation in pre-meiotic maize anthers
Transgenerational conditioned male fertility of HD-ZIP IV transcription factor mutant ocl4: impact on 21-nt phasiRNA accumulation in pre-meiotic maize anthers
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DOI：
10.1007/s00497-021-00406-3
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发表时间：
2021-03
影响因子：
3.4
通讯作者：
P. Yadava;Saleh Tamim;Han Zhang;Chong Teng;Xue Zhou;B. Meyers;V. Walbot
中科院分区：
文献类型：
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作者：
P. Yadava;Saleh Tamim;Han Zhang;Chong Teng;Xue Zhou;B. Meyers;V. Walbot
外细胞层4 （ocl4）编码一种HD-ZIP IV转录因子，这种转录因子是强大的男性生育能力和21-nt phasiRNA生物发生所必需的。在温暖的条件下，生育能力更有利，phasirna被部分恢复。环境敏感型雄性不育植物以前已经被描述过，可能是由不同的分子机制和生物学过程引起的，但假设环境条件下的跨代雄性生殖能力的恢复是一个相当新的谜团。在这里，我们报道了玉米雄性不育外细胞层4（ocl4）突变体的衍生系，该突变体的育性恢复并延续了几代。条件可育花药表现出部分复制的内胚层的解剖异常，就像它们的不育花药一样。我们分析了完全不育和不同等级的半不育花药在减数分裂前发育过程中的阶段性小干扰rna （phasiRNAs）的动态。条件可育花药积累的21-nt相rna显著高于无菌花药，表明条件可育花药中相rna的部分恢复。我们发现21-nt phasiRNAs的生物发生在很大程度上依赖于ocl4的三个关键步骤：(1)产生phas前体转录本，(2)调节前体加工的miR2118的表达，以及(3)21-nt phasiRNAs的积累。
Key messageMaizeOuter cell layer 4 (ocl4)encodes an HD-ZIP IV transcription factor required for robust male fertility and 21-nt phasiRNA biogenesis.ocl4fertility is favored in warm conditions, and phasiRNAs are partially restored.AbstractEnvironment-sensitive male-sterile plants have been described before and can result from different molecular mechanisms and biological processes, but putative environment-conditioned, transgenerational rescue of their male fertility is a rather new mystery. Here, we report a derivative line of the male-sterileouter cell layer 4(ocl4) mutant of maize, in which fertility was restored and perpetuated over several generations. Conditioned fertileocl4anthers exhibit the anatomical abnormality of a partially duplicated endothecial layer, just like their sterile counterparts. We profiled the dynamics of phased, small interfering RNAs (phasiRNAs) during pre-meiotic development in fully sterile and various grades of semi-fertileocl4anthers.The conditioned fertile anthers accumulated significantly higher 21-nt phasiRNAs compared toocl4sterile samples, suggesting a partial restoration of phasiRNAs in conditioned fertility. We found that the biogenesis of 21-nt phasiRNAs is largely dependent onOcl4at three key steps: (1) production ofPHASprecursor transcripts, (2) expression of miR2118 that modulates precursor processing, and (3) accumulation of 21-nt phasiRNAs.