EFFECTS OF INTRACRANIAL SELF-STIMULATION ON SELECTED PHYSIOLOGICAL VARIABLES IN RATS
EFFECTS OF INTRACRANIAL SELF-STIMULATION ON SELECTED PHYSIOLOGICAL VARIABLES IN RATS
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DOI：
10.1152/ajpregu.1993.264.1.r149
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发表时间：
1993-01-01
影响因子：
--
通讯作者：
BUGGY, J
中科院分区：
文献类型：
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作者：
BURGESS, ML;DAVIS, JM;BUGGY, J
本研究的目的是表征选定的代谢，心血管和激素反应，以加强颅内自我刺激（ICSS）的腹侧被盖区（VTA）的大鼠。20只雄性Sprague-Dawley大鼠立体定向植入双极电极，瞄准大脑的VTA。训练大鼠进行1周的ICSS训练。当它们通过坐在代谢操作室中适应实验环境时，它们连接到电极电缆，但不按压。所有动物均插有动脉导管。接受偶发刺激（C-St; n = 10）的大鼠在代谢操作室中进行30分钟的杠杆按压以加强脑刺激。氧耗量（VO 2）、心率（HR）、平均动脉压（MAP）和直肠温度（T（rec））随着应急脑刺激的开始和持续30 min而增加（P < 0.05）。此外，C-St大鼠血浆去甲肾上腺素（NE）、肾上腺素（Epi）和皮质酮显著高于静息值（P < 0.05）。5只动物接受了电刺激器递送的强化脑刺激（非相关刺激; NC-St），MAP、HR、VO 2、NE和Epi显著高于静息值（P < 0.05）。T（rec）和皮质酮对非偶然性脑刺激无反应。除HR外，非刺激对照组（n = 5）的任何测量变量均未出现高于静息值的增加。这些反应表明，偶然的脑刺激奖励会提高交感神经的兴奋程度。这些反应为使用ICSS作为行为动机的研究提供了重要信息，例如大鼠的体育锻炼，并将允许对动机行为的生理反应与单独ICSS发生的生理反应区分开来。
The purpose of this investigation was to characterize selected metabolic, cardiovascular, and hormonal responses to reinforcing intracranial self-stimulation (ICSS) of the ventral tegmental area (VTA) in rats. Twenty male Sprague-Dawley rats were stereotaxically implanted with bipolar electrodes aimed at the VTA of the brain. Rats were trained to lever-press for ICSS for 1 wk. While they adapted to the experimental environment by sitting in a metabolic operant chamber, they were connected to the electrode cable but did not lever-press. All animals were instrumented with arterial catheters. Rats receiving contingent stimulation (C-St; n = 10) performed 30 min of lever pressing in the metabolic operant chamber for reinforcing brain stimulation. Oxygen consumption (VO2), heart rate (HR), mean arterial pressure (MAP), and rectal temperature (T(rec)) increased with the onset and continuation of contingent brain stimulation over 30 min (P < 0.05). In addition, plasma norepinephrine (NE), epinephrine (Epi), and corticosterone increased significantly above resting values in C-St rats (P < 0.05). Five animals received investigator-delivered reinforcing brain stimulation (noncontingent stimulation; NC-St), with MAP, HR, VO2, NE, and Epi increasing significantly above resting values (P < 0.05). T(rec) and corticosterone were not responsive to noncontingent brain stimulation. With the exception of HR, nonstimulated controls (n = 5) did not experience increases above resting values in any of the variables measured. The responses suggest that contingent brain stimulation reward elicits heightened sympathetic arousal. These responses provide important information for studies that use ICSS as a motivator of behaviors, such as physical exercise in rats, and will allow the physiological responses to the motivated behaviors to be distinguished from those occurring from ICSS alone.