EVEN NUCLEIC ACIDS WITH 2',5'-LINKAGES FACILITATE DUPLEXES AND STRUCTURAL POLYMORPHISM : PROSPECTS OF 2',5'-OLIGONUCLEOTIDES AS ANTIGENE/ANTISENSE TOO L IN GENE REGULATION
EVEN NUCLEIC ACIDS WITH 2',5'-LINKAGES FACILITATE DUPLEXES AND STRUCTURAL POLYMORPHISM : PROSPECTS OF 2',5'-OLIGONUCLEOTIDES AS ANTIGENE/ANTISENSE TOO L IN GENE REGULATION
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即使具有 2,5-连接的核酸也有利于双链体和结构多态性: 2,5-寡核苷酸作为基因调控中的抗原/反义工具的前景
DOI：
10.1021/bi050013v
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发表时间：
1995
期刊：
影响因子：
1
通讯作者：
N. Yathindra
中科院分区：
文献类型：
--
作者：
V. Lalitha;N. Yathindra
我们已经制备了S核苷的2‘-氨基乙氧基衍生物(2AES)，并将其掺入三链形成的寡核苷酸中，用于识别靶标寡核苷酸中的TA中断。荧光熔融、紫外熔融和DNase I足迹实验表明，2AES对单个TA中断比G或S具有更强的亲和力。在pH 6.0时，在含有两个TA中断的18聚体靶位形成稳定的三联体，即使这包含八个C+.GC三联体。虽然2AES和S在TA中断时产生稳定的三链，但它们也与其他碱基对，特别是CG相互作用，尽管TA的选择性随着pH的增加而提高。2AES是迄今所描述的识别三螺旋靶标中TA的最佳核苷。
We have prepared the 2‘-aminoethoxy derivative of the S nucleoside (2AES) and incorporated it into triplex-forming oligonucleotides for recognition of TA interruptions within a target oligopurine tract. Fluorescence melting, UV melting, and DNase I footprinting experiments show that2AES has greater affinity than G or S for a single TA interruption. Stable triplexes are formed at pH 6.0 at an 18-mer target site containing two TA interruptions, even though this contains eight C+.GC triplets. Although2AES and S produce stable triplexes at TA interruptions, they also interact with other base pairs, in particular, CG, although the selectivity for TA improves with increased pH.2AES is the best nucleoside described so far for recognition of TA within a triple-helix target.