wingless signal and Zeste-white 3 kinase trigger opposing changes in the intracellular distribution of Armadillo.
wingless signal and Zeste-white 3 kinase trigger opposing changes in the intracellular distribution of Armadillo.
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发表时间：
1994-02
期刊：
影响因子：
4.6
通讯作者：
M. Peifer;D. Sweeton;Michael Casey;Eric Wieschaus
中科院分区：
文献类型：
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作者：
M. Peifer;D. Sweeton;Michael Casey;Eric Wieschaus
无翼/wnt-1信号在发育过程中指导细胞命运。果蝇的遗传分析鉴定出可能编码无翅信号转导系统成分的基因。果蝇犰狳是脊椎动物β -连环蛋白的同系物，是无翼信号所必需的。与犰狳RNA不同，犰狳蛋白在每个胚胎节段的不同细胞中不均匀地积累。我们发现，细胞在无翼信号的作用下改变了犰狳的细胞内分布，相对于膜相关蛋白，细胞质犰狳的积累水平增加。细胞质犰狳的水平也受Zeste-White 3激酶的调节。双突变体的分析表明，犰狳在无翼信号传导中的作用是直接的，并且犰狳在无翼和zete -white 3的下游都起作用。我们提出了犰狳条纹在无翼信号转导中的作用模型。
wingless/wnt-1 signaling directs cell fate during development. Genetic analysis in Drosophila identified genes that may encode components of the wingless signal transduction system. Drosophila Armadillo, homolog of vertebrate beta-catenin, is required for wingless signaling. Unlike armadillo RNA, Armadillo protein accumulates non-uniformly in different cells of each embryonic segment. We found that cells alter their intracellular distribution of Armadillo in response to Wingless signal, accumulating increased levels of cytoplasmic Armadillo relative to those of membrane-associated protein. Levels of cytoplasmic Armadillo are also regulated by Zeste-White 3 kinase. Analysis of double mutants demonstrates that Armadillo's role in wingless signaling is direct, and that Armadillo functions downstream of both wingless and zeste-white 3. We present a model for the role of Armadillo stripes in transduction of wingless signal.