Connections of indoleamine‐accumulating cells in the rabbit retina
Connections of indoleamine‐accumulating cells in the rabbit retina
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兔视网膜中吲哚胺累积细胞的连接
DOI：
10.1002/cne.902830210
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发表时间：
1989
影响因子：
2.5
通讯作者：
R. Dacheux
中科院分区：
文献类型：
--
作者：
J. H. Sandell;R. Masland;E. Raviola;R. Dacheux
为了研究兔视网膜中积聚吲哚胺的神经元之间的联系，我们将5，7-二羟色胺注入玻璃体。它聚集在无长突细胞的一个亚群中，并可以通过乙醛诱导的荧光在那里观察到。在二氨基联胺的存在下，荧光标记被光转化为不溶于水的嗜Os产物，并用电子显微镜观察组织。光转化后组织结构的保存令人满意，吲哚胺蓄积细胞的树突也很容易辨认。它们在内丛细胞层和神经节细胞层的交界处形成致密的神经丛，在那里它们显示出巨大的突触终末，占据了视杆双极终末表面的很大一部分。吲哚胺蓄积细胞的树突接受来自二联体突触的杆状双极的输入，而另一个突触后伙伴是窄视野的双层无长突细胞的树突；此外，它们接受内丛状层的无长突细胞的输入。我们观察到的唯一输出是视杆双极末端的相互突触。因此，这些无长突细胞似乎在通过视网膜传递暗视信息方面发挥着重要作用。
To study the connections of the neurons of the rabbit retina that accumulate indoleamines, we injected 5,7‐dihydroxytryptamine into the vitreous body. It accumulated within a subset of amacrine cells and could be visualized there by aldehyde‐induced fluorescence. The fluorescent labeling was photoconverted to an insoluble, osmiophilic product by irradiation in the presence of diaminobenzidine, and the tissue was examined by electron microscopy. Preservation of the structure of the tissue after photoconversion was satisfactory and the dendrites of the indoleamine‐accumulating cells could easily be identified. They form a dense plexus near the junction of the inner plexiform and ganglion cell layers, where they exhibit large synaptic endings that occupy a substantial fraction of the surface of rod bipolar terminals. The dendrites of the indoleamine‐accumulating cells receive input from rod bipolars at dyad synapses, where the other postsynaptic partner is a dendrite of a narrow‐field, bistratified amacrine cell; in addition, they receive amacrine cell input throughout the inner plexiform layer. The only outputs we observed are reciprocal synapses onto the rod bipolar endings. Thus, these amacrine cells appear to exert an important effect on the transmission of scotopic information through the retina.