Multilocus sequence typing and variations in the oprD gene of Pseudomonas aeruginosa isolated from a hospital in China.
Multilocus sequence typing and variations in the oprD gene of Pseudomonas aeruginosa isolated from a hospital in China.
复制标题
中国某医院铜绿假单胞菌oprD基因多位点序列分型及变异
DOI：
10.2147/idr.s152162
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发表时间：
2018
影响因子：
3.9
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Liu H;Kong W;Yang W;Chen G;Liang H;Zhang Y
目的 提供中国某医院临床分离铜绿假单胞菌碳青霉烯类耐药的遗传关系和机制信息。材料和方法 从中国一家医院分离出一百六十株铜绿假单胞菌菌株。通过抗菌药物敏感性测试确定对 14 种抗菌药物的敏感性。使用多位点序列分型来表征这些临床分离株的遗传背景。随机选取45株菌株用于进一步评估其碳青霉烯类耐药机制。他们的 oprD 基因与 PAO1 序列进行了比较。结果多位点序列分型分析表明这些分离株具有高度多样性；鉴定出 68 种序列类型，其中 28 种为新序列类型。多基因和 eBURST 分析证明了基因相似的克隆具有不同的抗性特征。随机选取的45株与碳青霉烯类耐药相关的菌株中，2株为金属β-内酰胺酶产生菌；所有 45 个菌株均不是 AmpC 过量生产者。序列分析显示分离株之间的 oprD 序列具有高度多样性。 oprD中L7和L8环缩短的对亚胺培南和美罗培南敏感的菌株是该院观察到的主要菌株类型。结论本研究表明oprD提供了碳青霉烯类耐药的主要机制。缩短的 L7 和 L8 环是造成碳青霉烯类药物敏感性的原因。
Objectives To provide information about the genetic relationships and mechanism underlying carbapenem resistance in Pseudomonas aeruginosa clinical isolates of a hospital in China. Materials and methods One hundred and sixty P. aeruginosa strains were isolated from a hospital in China. Susceptibility to 14 antimicrobial agents was determined by antimicrobial susceptibility testing. Multilocus sequence typing was used to characterize the genetic backgrounds of these clinical isolates. Forty-five strains were randomly selected for further evaluation of their carbapenem resistance mechanism. Their oprD gene was compared with the PAO1 sequence. Results Multilocus sequence typing analysis demonstrated that these isolates were highly diverse; 68 sequence types were identified, of which 28 were novel sequence types. Polygenic and eBURST analysis demonstrated genetically similar clones with dissimilar resistance profiles. Among the 45 randomly selected strains associated with carbapenem resistance, 2 were metallo β-lactamase producers; all the 45 strains were not AmpC overproducers. Sequence analysis revealed a high diversity in the oprD sequences among isolates. Strains susceptible to imipenem and meropenem with shortened L7 and L8 loops in oprD were the major strain types observed in this hospital. Conclusion This study indicated that oprD provided the main mechanism for carbapenem resistance. The shortened L7 and L8 loops are responsible for carbapenem susceptibility.