Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
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DOI：
10.3791/2844
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发表时间：
2011-07-01
影响因子：
1.2
通讯作者：
Allen, Matthew J.
中科院分区：
文献类型：
--
作者：
Averill, Derek J.;Garcia, Joel;Allen, Matthew J.
基于多氨基多羧酸盐的配体通常用于螯合镧系元素离子，并且所得络合物可用作磁共振成像（MRI）的造影剂。许多市售配体是特别有用的，因为它们含有允许通过胺反应性活化酯和异硫氰酸酯基团或硫醇反应性马来酰亚胺与大分子和生物分子快速、高纯度和高产率缀合的官能团。虽然这些配体的金属化被认为是生物缀合化学领域的常识，但在选择金属起始材料时必须考虑金属化过程中的细微差异。此外，存在多种纯化和表征选择，并且最有效程序的选择部分取决于起始材料的选择。这些细微的差异在已发表的协议中经常被忽略。在这里，我们的目标是展示金属化，纯化和表征镧系元素络合物的常用方法，这些络合物可用作MRI造影剂（图1）。我们希望，这篇文章将使生物医学科学家通过简化起始材料和纯化方法的选择，将镧系元素络合反应纳入他们常用的反应体系。
Polyaminopolycarboxylate-based ligands are commonly used to chelate lanthanide ions, and the resulting complexes are useful as contrast agents for magnetic resonance imaging (MRI). Many commercially available ligands are especially useful because they contain functional groups that allow for fast, high-purity, and high-yielding conjugation to macromolecules and biomolecules via amine-reactive activated esters and isothiocyanate groups or thiol-reactive maleimides. While metalation of these ligands is considered common knowledge in the field of bioconjugation chemistry, subtle differences in metalation procedures must be taken into account when selecting metal starting materials. Furthermore, multiple options for purification and characterization exist, and selection of the most effective procedure partially depends on the selection of starting materials. These subtle differences are often neglected in published protocols. Here, our goal is to demonstrate common methods for metalation, purification, and characterization of lanthanide complexes that can be used as contrast agents for MRI (Figure 1). We expect that this publication will enable biomedical scientists to incorporate lanthanide complexation reactions into their repertoire of commonly used reactions by easing the selection of starting materials and purification methods.