Chronic consumption of a low-fat diet leads to increased hypothalamic agouti-related protein and reduced leptin.
Chronic consumption of a low-fat diet leads to increased hypothalamic agouti-related protein and reduced leptin.
复制标题
长期食用低脂饮食会导致下丘脑刺鼠相关蛋白增加和瘦素减少。
DOI：
10.1016/j.nut.2007.06.001
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Argyropoulos,George
中科院分区：
文献类型：
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作者：
Staszkiewicz,Jaroslaw;Horswell,Ronald;Argyropoulos,George
本研究验证了在自由采食条件下膳食脂肪影响小鼠发育早期AgRP、瘦素、瘦素受体（OBRb）和神经肽Y（NPY）表达水平的假设。方法将雄性SC 57 Bl/6 J小鼠断奶后不久分别给予高脂饮食（HFD）或低脂饮食（LFD）。各组小鼠在不同年龄被安乐死，实时一步逆转录聚合酶链反应被用来分析基因表达在下丘脑（AgRP，NPY，OBRb），肾上腺（AgRP），睾丸（AgRP），和附睾脂肪（瘦素）。这种表达模式与HFD下下丘脑AgRP的表达减少相吻合，而OBRb和NPY在饮食中没有波动。相比之下，LFD的消耗（即，高碳水化合物）增加下丘脑AgRP和抑制脂肪瘦素，这与瘦素可中枢调节AgRP的观点一致。相比之下，AgRP在肾上腺中的表达最初下降，然后随着年龄的增长，在这两种diets. CONCLUSIONSDeitary脂肪可以有组织依赖性的影响AgRP，可能是不受约束的瘦素下HFD。
OBJECTIVEThis study examined the hypothesis that dietary fat under ad libitum feeding conditions influences expression levels (mRNA) of the mouse agouti-related protein (AgRP), leptin, leptin receptor (OBRb), and neuropeptide Y (NPY) at early stages of development.METHODSC57Bl/6J male mice were placed on a high-fat diet (HFD) or a low-fat diet (LFD) shortly after weaning. Groups of mice were euthanized at various ages and real-time one-step reverse transcriptase polymerase chain reaction was used to analyze gene expression in the hypothalamus (AgRP, NPY, OBRb), the adrenal gland (AgRP), the testis (AgRP), and epididymal fat (leptin).RESULTSLeptin expression increased linearly with age but only under the HFD despite body weight gain under both diets. This pattern of expression coincided with reduced expression of hypothalamic AgRP under an HFD, whereas OBRb and NPY did not fluctuate in response to diet. By contrast, consumption of an LFD (i.e., high carbohydrate) increased hypothalamic AgRP and suppressed adipose leptin, which is consistent with the notion that leptin could regulate AgRP centrally. In contrast, AgRP expression in the adrenal gland initially decreased and then increased with age under both diets.CONCLUSIONSDietary fat can have a tissue-dependent effect on AgRP that may be unfettered by leptin under an HFD.