Altered Expression of Inducible Nitric Oxide Synthase After Sciatic Nerve Injury in Rat
Altered Expression of Inducible Nitric Oxide Synthase After Sciatic Nerve Injury in Rat
复制标题
大鼠坐骨神经损伤后诱导型一氧化氮合酶表达的改变
DOI：
10.1007/s12013-011-9192-6
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发表时间：
2011-11-01
影响因子：
2.6
通讯作者：
Chen, Desong
中科院分区：
文献类型：
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作者：
Lin, Haodong;Hou, Chunlin;Chen, Desong
一氧化氮被认为是神经元损伤和损伤后外周神经元再生的重要因素。坐骨神经损伤是肌肉注射的常见和严重并发症。为了探讨诱导型一氧化氮合酶(INOS)在坐骨神经损伤中的作用，我们用RT-PCR和免疫组织化学方法研究了诱导型一氧化氮合酶在坐骨神经损伤模型中的表达。在假手术对照组大鼠，免疫组织化学检测不到iNOS的表达，其mRNA水平也很低。相反，在受到坐骨神经损伤的实验组中，iNOS的mRNA和蛋白都被发现显著升高。免疫组织化学阳性染色显示，iNOS蛋白水平在术后7d达到高峰，随后下降。同样，iNOS mRNA水平在术后第1、3、7天仍保持较高水平，但到第21天下降至非常低的水平。提示大鼠坐骨神经损伤后诱导型一氧化氮合酶表达增加，可能与神经再生有关。因此，我们的结果提示，神经损伤后iNOS的过度表达不利于神经再生。
Nitric oxide is known to contribute to neuronal damage as well as to peripheral neuronal regeneration following injury. Sciatic nerve injury is a common and serious complication of intramuscular injections. In order to ascertain the role of inducible nitric oxide synthase (iNOS) in the injured sciatic nerve, we studied the expression of this enzyme by RT-PCR and immunohistochemistry, in a rat model of sciatic nerve injury. In sham-operated control rats iNOS expression was undetectable by immunohistochemistry and its mRNA level was also very low. In contrast, in the experimental group that was subjected to sciatic nerve injury, both mRNA and protein of iNOS were found to be significantly elevated. The protein level of iNOS, as revealed by positive immunostaining, peaked at 7 days post-surgery followed by a decrease. Similarly, the iNOS mRNA levels remained elevated at 1, 3, 7 days but declined to very low level by day 21, after surgery. This study indicates that the increased expression of iNOS after sciatic nerve injury in rats may contribute to nerve regeneration. Thus our results suggest that excessive expression of iNOS after nerve injury is not conducive to nerve regeneration.