Lectin-modified trifunctional nanobiosensors for mapping cell surface glycoconjugates
Lectin-modified trifunctional nanobiosensors for mapping cell surface glycoconjugates
复制标题
凝集素修饰的三功能纳米生物传感器用于绘制细胞表面糖缀合物
DOI：
10.1016/j.bios.2008.07.058
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发表时间：
2009-01-01
影响因子：
12.6
通讯作者：
Pang, Dai-Wen
中科院分区：
文献类型：
--
作者：
Xie, Min;Hu, Jun;Pang, Dai-Wen
基于纳米材料的纳米生物传感器（nanobiodevices或nanobioprobes）越来越受到重视。在此，将量子点和γ-Fe 2 O3磁性纳米粒子共嵌入单溶胀聚（苯乙烯/丙烯酰胺）共聚物纳米球中，制备荧光-磁性双功能纳米球。随后，方便地制备了用小麦胚芽凝集素（WGA）、花生凝集素（PNA）或双花扁豆凝集素（DBA）修饰的荧光-磁性-生物靶向三功能纳米生物传感器（TFNS），以便与表面表达有N-乙酰氨基葡萄糖、D-半乳糖和N-乙酰氨基半乳糖残基的A549细胞结合。计算每个纳米生物传感器上的WGA、PNA和DBA的值分别为40、14和60。这三种凝集素修饰的三功能纳米生物传感器（lectin-TFNS）可用于A549细胞表面糖复合物的定性和定量分析。在A549细胞表面，WGA修饰的N-乙酰氨基葡萄糖纳米生物传感器的荧光强度明显高于PNA修饰的D-半乳糖胺纳米生物传感器和DBA修饰的N-乙酰氨基葡萄糖纳米生物传感器，这与流式细胞仪检测结果一致。凝集素修饰的三功能纳米生物传感器不仅可以对A549细胞表面的糖复合物进行定量分析，而且可以识别和分离A549细胞。0.5 5 mg的WGA修饰的荧光-磁性三功能纳米生物传感器可以捕获7.0 × 10（4）个A549细胞。因此，凝集素修饰的三功能纳米生物传感器可以应用于在细胞表面的糖缀合物的映射和用于识别和分离靶细胞。(C)2008 Elsevier B. V.保留所有权利。
Nanomaterial-based nanobiosensors (nanobiodevices or nanobioprobes) are increasingly emphasized. Here, quantum dots and gamma-Fe2O3 magnetic nanoparticles were co-embedded into single swelling poly(styrene/acrylamide) copolymer nanospheres to fabricate fluorescent-magnetic bifunctional nanospheres. Subsequently, fluorescent-magnetic-biotargeting trifunctional nanobiosensors (TFNS) modified with wheat germ agglutinin (WGA), peanut agglutinin (PNA) or Dolichos biflorus agglutinin (DBA) were conveniently produced so as to bind with A549 cells which are surface-ex pressed with N-acetylglucosamine, D-galactosamine and N-acetylgalactosamine residues. The values of WGA, PNA and DBA on each nanobiosensor were calculated to be 40, 14 and 60, respectively. These three kinds of lectin-modified trifunctional nanobiosensors (lectin-TFNS) can be used for qualitative and quantitative analysis of the glycoconjugates on A549 cell Surface. The fluorescence intensity of WGA-modified nanobiosensors related to N-acetylglucosamine on A549 cell surface was Much higher than that of PNA-modified nanobiosensors corresponding to D-galactosamine and that of N-acetylgalactosamine-related DBA-modified nanobiosensors, which is consistent with the results detected by flow cytometry. Lectin-modified trifunctional nanobiosensors not only can quantify the different glycoconjugates on A549 cell surface, but also can recognize and isolate A549 cells. 0.5 mg of WGA-modified fluorescent-magnetic trifunctional nanobiosensors could capture 7.0 x 10(4) A549 cells. Therefore, the lectin-modified trifunctional nanobiosensors may be applied in mapping the glycoconjugates on cell surfaces and for recognition and isolation of targeted cells. (C) 2008 Elsevier B.V. All rights reserved.