The histone deacetylase 1/GSK3/SHAGGY-like kinase 2/BRASSINAZOLE-RESISTANT 1 module controls lateral root formation in rice
The histone deacetylase 1/GSK3/SHAGGY-like kinase 2/BRASSINAZOLE-RESISTANT 1 module controls lateral root formation in rice
复制标题
组蛋白脱乙酰酶 1/GSK3/SHAGGY 样激酶 2/BRASSINAZOLE-RESISTANT 1 模块控制水稻侧根形成
DOI：
10.1093/plphys/kiac015
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发表时间：
2022-06-01
期刊：
影响因子：
7.4
通讯作者：
Li, Lijia
中科院分区：
文献类型：
--
作者：
Hou, Jiaqi;Zheng, Xueke;Li, Lijia
侧根(LRs)是水稻根系的主要组成部分，它增加了根表面积，使其能够有效地吸收水分和养分。然而，调控水稻LR形成的分子机制在很大程度上仍不清楚。在此，我们报道了组蛋白脱乙酰酶1(OsHDAC1)正向调节水稻LR的形成。水稻OsHDAC1 RNAi植株比野生型植株产生更少的LR，而过量表达OsHDAC1的植株通过促进LR原基的形成而促进LR的增殖。油菜素类固醇处理增加了LR数，GSK3/Saggy-like kinase2(OsGSK2)突变也增加了LR数，而过表达OsGSK2则减少了LR数。重要的是，OsHDAC1可以直接与OsGSK2相互作用并使其去乙酰化，从而抑制其活性。OsGSK2去乙酰化减弱了OsGSK2与BRASSINAZOLE抗性基因1(OsBZR1)之间的相互作用，导致OsBZR1的积累。OsBZR1的过表达通过调节生长素/IAA信号基因促进了LR的形成。综上所述，这些结果表明，OsHDAC1通过失活OsGSK2来调节水稻LR的形成，从而阻止LR原基形成的正调控因子OsBZR1的降解。我们的发现表明OsHDAC1是一个可以提高水稻资源捕获的育种靶标。组蛋白脱乙酰酶1介导油菜素类固醇信号成分控制水稻侧根形成，这阐明了分子设计育种的调控机制。
Lateral roots (LRs) are a main component of the root system of rice (Oryza sativa) that increases root surface area, enabling efficient absorption of water and nutrients. However, the molecular mechanism regulating LR formation in rice remains largely unknown. Here, we report that histone deacetylase 1 (OsHDAC1) positively regulates LR formation in rice. Rice OsHDAC1 RNAi plants produced fewer LRs than wild-type plants, whereas plants overexpressing OsHDAC1 exhibited increased LR proliferation by promoting LR primordia formation. Brassinosteroid treatment increased the LR number, as did mutation of GSK3/SHAGGY-like kinase 2 (OsGSK2), whereas overexpression of OsGSK2 decreased the LR number. Importantly, OsHDAC1 could directly interact with and deacetylate OsGSK2, inhibiting its activity. OsGSK2 deacetylation attenuated the interaction between OsGSK2 and BRASSINAZOLE-RESISTANT 1 (OsBZR1), leading to accumulation of OsBZR1. The overexpression of OsBZR1 increased LR formation by regulating Auxin/IAA signaling genes. Taken together, the results indicate that OsHDAC1 regulates LR formation in rice by deactivating OsGSK2, thereby preventing degradation of OsBZR1, a positive regulator of LR primordia formation. Our findings suggest that OsHDAC1 is a breeding target in rice that can improve resource capture.Histone deacetylase 1 mediates brassinosteroid signaling components to control rice lateral root formation, which elucidates a regulatory mechanism for molecular design breeding.