Immobilization of highly concentrated cell suspensions using the laminar jet breakup technique
Immobilization of highly concentrated cell suspensions using the laminar jet breakup technique
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DOI：
10.1021/bp990033m
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发表时间：
1999-05-01
影响因子：
2.9
通讯作者：
Widmer, F
中科院分区：
文献类型：
--
作者：
Brandenberger, HR;Widmer, F
扰动射流的受控破碎是用于单分散颗粒生产的公知技术，并且已经针对牛顿流体和粘弹性流体进行了充分的研究。对于将细胞固定在水凝胶的珠粒中，已经观察到存在最大细胞浓度，超过该最大细胞浓度不能达到单分散珠粒分布。更高的浓度导致不规则的射流破碎，从而导致液滴聚结形成具有两倍或三倍体积的珠粒。使用不同直径的乳胶珠作为细胞的模型物质和面包酵母作为比较，研究了不同操作条件下的最大细胞浓度。使用静电液滴分散系统增加了仍然可以实现单分散尺寸分布的最大细胞浓度。
The controlled breakup of a disturbed jet is a well-known technique for monodisperse particle production and has been well-investigated for Newtonian and viscoelastic fluids. For the immobilization of cells in beads of a hydrogel it has been observed that there is a maximum cell concentration beyond which a monodisperse bead distribution cannot be reached. Higher concentrations lead to irregular jet breakup and thus to droplet coalescence forming beads with double or triple volume. This maximum cell concentration has been investigated for different operating conditions using latex beads of different diameters as a model substance for cells and baker's yeast as a comparison. The maximum cell concentration where a monodisperse size distribution could still be achieved was increased using an electrostatic droplet dispersion system.