Characterization of reactive oxygen species generated by protoporphyrin IX under X-ray irradiation
Characterization of reactive oxygen species generated by protoporphyrin IX under X-ray irradiation
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DOI：
10.1016/j.radphyschem.2009.06.036
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发表时间：
2009-11-01
影响因子：
2.9
通讯作者：
Misawa, Masaki
中科院分区：
文献类型：
--
作者：
Takahashi, Junko;Misawa, Masaki
作为一种可能的具有生物相容性、致癌性和X射线激活能力的辐射增敏剂，考察了原卟啉IX(PpIX)在X射线和紫外线照射下对促进活性氧(ROS)生成的贡献。用2-[6-(4-氨基)苯氧基-~3H-黄原酮-3-on-9-基]苯甲酸(APF)和二氢乙锭(DHE)与乙醇作为羟基自由基(OH中心点)猝灭剂，对ROS的种类进行了鉴定。在X射线和紫外光照射下，除了辐射和光解外，PpIX还增强了我们感兴趣的三种物种(OH中心点、超氧自由基(O-2(中心点-))和单线态氧(O-1(2)。它的增强系数超过1.7，取决于PpIX的浓度从1.5到15.0微克/毫升。(C)2009爱思唯尔有限公司。保留所有权利。
As a possible radiosensitizer candidate having biological compatibility, oncotropic property, and X-ray activation capability, contribution of protoporphyrin IX (PpIX) to enhanced generation of reactive oxygen species (ROS) under X-ray and UV irradiations were examined. To identify the kinds of ROS, 2-[6-(4-amino)phenoxy-3H-xanthen-3-on-9-yl] benzoic acid (APF) and dihydroethidium (DHE) were used together with ethanol as a hydroxyl radical (OH center dot) quencher. All of the three species of our interest (OH center dot, superoxide radical (O-2(center dot-)), and singlet oxygen (O-1(2))) were enhanced by PpIX under X-ray and UV irradiations in addition to those by radiolysis and photolysis. Its enhancement factors exceeded 1.7 depending on the concentrations of PpIX from 1.5 to 15.0 mu g/ml. (C) 2009 Elsevier Ltd. All rights reserved.