Structural characterization of the N-linked glycans from Taenia solium metacestodes
Structural characterization of the N-linked glycans from Taenia solium metacestodes
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DOI：
10.1016/s0166-6851(02)00250-5
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发表时间：
2003-01-01
影响因子：
1.5
通讯作者：
Dell, A
中科院分区：
文献类型：
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作者：
Haslam, SM;Restrepo, BI;Dell, A
脑囊虫病(NCC)是由猪带绦虫囊虫引起的一种危及生命的中枢神经系统(CNS)感染。这种疾病表现出非特异性的体征和症状，如癫痫发作和颅内压升高，因此很容易与其他中枢神经系统病变相混淆[1]。对与凝集素Lens culinaris有亲和力的糖蛋白的广泛研究表明，这些分子具有高度的抗原性和特异性，适合于NCC的血清学诊断[2-4]。然而，到目前为止，这些分子还没有成功地在重组表达系统中复制出来进行大规模生产。迄今为止报道的重组蛋白或合成肽的抗原性较差，至少部分原因是碳水化合物对其免疫原性的贡献。我们对12、16、18、24和28 kDa的猪带绦虫糖蛋白(TSG)的研究表明，N-连接的碳水化合物有助于所有这些蛋白的抗原性，但对18 kDa的分子[4，7]更为显著；缩写：FAB-，快原子轰击-；FUC，岩藻糖；Gal，半乳糖；GalNAc，N-乙酰半乳糖胺；GlcNAc，N-乙酰氨基葡萄糖；Hex，己糖；HexNAc，N-乙酰氨基己糖；人，甘露糖；MS，光谱分析；NCC，神经囊虫病；CNS，中枢神经系统；PNGA，N-糖苷酶；PNGF酶，肽
Neurocysticercosis (NCC) is a life-threatening central nervous system (CNS) infection caused by the metacestode or cysticercus of the flatworm Taenia solium. This disease presents non-specific signs and symptoms like seizures and increased intracranial pressure, and therefore can be easily confused with other CNS pathologies [1]. Extensive studies of glycoproteins with affinity for the lectin Lens culinaris have indicated that these molecules are appropriate for the serological diagnosis of NCC, given their high antigenicity and specificity [2–4]. However, to date these molecules have not been successfully reproduced in recombinant expression systems for their large-scale production. The poor antigenicity of the recombinant proteins, or synthetic peptides reported to date [5, 6], could be explained at least in part by the contribution of carbohydrates towards their immunogenicity. Our studies with the Taenia solium glycoproteins (TSGs) of 12, 16, 18, 24 and 28 kDa suggest that the N-linked carbohydrates contribute to the antigenicity of all of them, but is more notable for the 18 kDa molecule [4, 7]; Abbreviations: FAB-, fast atom bombardment-; Fuc, fucose; Gal, galactose; GalNAc, N-acetylgalactosamine; GlcNAc, N-acetylglucosamine; Hex, hexose; HexNAc, N-acetylhexosamine; Man, mannose; MS, mass spectrometry; NCC, neurocysticercosis; CNS, central nervous system; PNGase A, peptide N-glycosidase A; PNGase F, peptide