Molecular characterization of parthenogenic Fasciola sp in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene
Molecular characterization of parthenogenic Fasciola sp in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene
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DOI：
10.1292/jvms.67.1115
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发表时间：
2005-11-01
影响因子：
1.2
通讯作者：
Fukuda, K
中科院分区：
文献类型：
--
作者：
Itagaki, T;Kikawa, M;Fukuda, K
核糖体内转录间隔区（ITS 1）和线粒体NADH脱氢酶I（NDI）基因的核苷酸序列进行了分析，在韩国的无精子片形吸虫的遗传特征。根据ITS I序列的差异，将韩国吸虫分为Kor 1、Kor 2和Kor 1/2 3种单倍型，它们与F. hepatica、F.结果表明，两种植物的种间差异不显著，种间重叠的种间差异显著。NDI序列还显示，韩国吸虫可分为3种不同的单倍型（Kor 1：F. hepatica-type，Kor2a和Kor2b：F.南极型）。Kor 1和Kor 2a的序列分别与日本主要片形吸虫的单倍型Fsp 1和Fsp 2的序列100%相同。这些发现强烈表明，在韩国和日本的无精子片形吸虫起源于相同的祖先，最近在这两个国家蔓延。
Nucleotide sequences of ribosomal internal transcribed spacer (ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea. From the difference in ITS I sequences, Korean flukes were divided into 3 haplotypes represented by Kor1, Kor2 and Kor1/2, which had nucleotides identical to F. hepatica, F. gigantica and those overlapped between the two species, respectively. NDI sequences also showed that Korean flukes could be classified into 3 distinct haplotypes (Kor1: F. hepatica-type, Kor2a and Kor2b: F. gigantica-type). The sequences of Kor1 and Kor2a were 100% identical to those of the haplotypes Fsp1 and Fsp2, respectively, which are major Fasciola forms in Japan. These findings strongly suggest that aspermic Fasciola forms in Korea and Japan originated from same ancestors and have recently spread throughout both countries.