Insulin antagonism of dexamethasone-induced stimulation of cholinephosphate cytidylyltransferase in fetal rat lung in organ culture.
Insulin antagonism of dexamethasone-induced stimulation of cholinephosphate cytidylyltransferase in fetal rat lung in organ culture.
复制标题
器官培养中胎鼠肺中地塞米松诱导的磷酸胆碱胞苷酰转移酶刺激的胰岛素拮抗作用。
作者：
Rooney,SA;Ingleson,LD;Wilson,CM;Gross,I
本文研究了胰岛素和地塞米松对器官培养胎鼠肺组织中磷脂酰胆碱合成的拮抗作用。取18d胎鼠肺组织，在含有10−、6M地塞米松、1U/ml牛胰岛素或两者同时存在的无血清培养液中培养48h，对照组在无激素条件下培养48h。单用地塞米松对磷酸胆碱胞苷转移酶的活性有134%的刺激作用，而单用胰岛素则无此作用。然而，当胰岛素和地塞米松一起加入时，刺激性降低到72%。提示胰岛素对地塞米松刺激胎肺磷脂酰胆碱生物合成的拮抗作用可能至少部分在胆碱磷酸胞苷转移酶水平上表达。
We have studied the antagonism between insulin and dexamethasone on phosphatidylcholine synthesis in fetal rat lung in organ culture. Explants from 18-day fetal rat lungs were cultured in serum-free medium in the presence of 10−6M dexamethasone phosphate, 1 U/ml bovine insulin or both hormones for 48 h. Control explants were similarly cultured in the absence of hormones. Dexamethasone alone stimulated the activity of cholinephosphate cytidylyltransferase by 134% while insulin alone had no effect. When insulin was added together with dexamethasone, however, the stimulation was reduced to 72%. These data suggest that antagonism by insulin of dexamethasone-induced stimulation of fetal lung phosphatidylcholine biosynthesis may be at least partially expressed at the level of cholinephosphate cytidylyltransferase.