Factors influencing elevated ethanol consumption in adolescent relative to adult rats
Factors influencing elevated ethanol consumption in adolescent relative to adult rats
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DOI：
10.1097/01.alc.0000183007.65998.aa
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发表时间：
2005-10-01
影响因子：
3.2
通讯作者：
Spear, LP
中科院分区：
文献类型：
--
作者：
Doremus, TL;Brunell, SC;Spear, LP
背景：许多青少年报告在青春期经常饮酒。动物模型可能是一个重要的工具，探索因素，有助于酒精消费在这一关键时期。方法：使用24小时，免费访问，两瓶之间的水和加糖溶液或不加乙醇的非剥夺大鼠的选择程序，本系列的实验研究了各种背景和实验变量的贡献（即，单独饲养与成对饲养、吸管类型、溶液的热量值、之前的实验干扰）对青春期和成年Sprague-Dawley大鼠的酒精消耗量的影响。结果：使用含滚珠轴承的圆珠笔（BP）吸管的青春期大鼠的酒精消耗量特别大，这加剧了摄入量，而不是由于乙醇溶液的热量含量。隔离住房12天并没有改变酒精消费的青少年相对于他们的社会住房的同行，同时抑制消费的孤立的成年人。对本系列实验中青少年乙醇消耗量相对大小的差异进行的检查显示，青少年的乙醇摄入量不仅因纳入BP吸管而升高，而且还因相对于新型乙醇溶液的出现错开分离物圈养的时间而升高。该实验系列的结果表明，在各种饲养笼、连续进入环境下，青少年动物消耗的乙醇明显多于成年动物，由于青少年的摄入量相对较大，一些测试条件进一步放大了这一点。通常被认为是无害的微妙的实验细节可能对在青少年和成年动物中观察到的自愿乙醇消耗总量产生重大影响。
Background: Many teenagers report frequent alcohol use during the adolescent period. Animal models may be an important tool for exploring factors that contribute to alcohol consumption during this critical period.Methods: Using a 24-hour, free-access, two-bottle-choice procedure between water and a sweetened solution with or without ethanol in nondeprived rats, the present series of experiments examined the contribution of a variety of contextual and experimental variables (i.e., isolate-housing versus pair-housing, type of sipper tube, caloric value of solution, prior experimental perturbations) on alcohol consumption in both adolescent and adult Sprague-Dawley rats.Results: Ethanol consumption was particularly magnified among adolescent rats using ball bearing-containing ball-point (BP) sipper tubes, with this exacerbated intake not due to caloric content of the ethanol solution. Isolation housing for 12 days did not alter ethanol consumption of adolescents relative to their socially housed counterparts while suppressing consumption of isolated adults. An examination of differences in the relative magnitude of adolescent ethanol consumption across experiments in this series revealed that ethanol intake among adolescents was elevated not only by the inclusion of BP sipper tubes but also by staggering the timing of isolate housing relative to the presentation of the novel ethanol solution.Conclusions: The results of this experimental series demonstrate that adolescent animals consume significantly more ethanol than adult animals under a variety of home cage, continuous-access circumstances, with the relatively greater intake of adolescents further magnified by a number of test conditions. Subtle experimental details often thought to be innocuous can have a substantial impact on overall amount of voluntary ethanol consumption observed in both adolescent and adult animals.