Toyo virus, a novel member of the Kaisodi group in the genus Uukuvirus (family Phenuiviridae) found in Haemaphysalis formosensis ticks in Japan
Toyo virus, a novel member of the Kaisodi group in the genus Uukuvirus (family Phenuiviridae) found in Haemaphysalis formosensis ticks in Japan
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DOI：
10.1007/s00705-021-05193-w
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发表时间：
2021-08-02
影响因子：
2.7
通讯作者：
Isawa, Haruhiko
中科院分区：
文献类型：
--
作者：
Kobayashi, Daisuke;Kuwata, Ryusei;Isawa, Haruhiko
扁虱是一种重要的媒介节肢动物，可将各种病原体传播给人类和其他动物。壁虱传播的病毒对公众健康尤其令人担忧，因为它们是新出现和再次出现的传染病的主要病原体。硬蜱传播病毒的菲尼韦科病毒家族是种类最多的一组，包括重要的人类致病病毒，如严重发热伴血小板减少综合征病毒。在使用RNA病毒体分析从日本爱媛采集的台湾血蜱样本中检测到硬蜱传播病毒的监测期间，检测到了类似于PhenuiVirus的序列。RT-PCR扩增和Sanger测序显示了所有三个片段的几乎完整的病毒基因组序列。病毒的氨基酸序列比较表明，检测到的病毒与乌库病毒属Kaisodi组的已知成员有46%-70%的序列同源性。此外，病毒蛋白的系统发育分析表明，该病毒与Kaisodi群病毒形成了一个簇，表明这是一个新的病毒，命名为东洋病毒(TOYOV)。需要对TOYOV进行进一步的研究，这将有助于了解Kaisodi组病毒的自然历史和病原学重要性。
Ticks are important vector arthropods that transmit various pathogens to humans and other animals. Tick-borne viruses are of particular concern to public health as these are major agents of emerging and re-emerging infectious diseases. The Phenuiviridae family of tick-borne viruses is one of the most diverse groups and includes important human pathogenic viruses such as severe fever with thrombocytopenia syndrome virus. Phenuivirus-like sequences were detected during the surveillance of tick-borne viruses using RNA virome analysis from a pooled sample of Haemaphysalis formosensis ticks collected in Ehime, Japan. RT-PCR amplification and Sanger sequencing revealed the nearly complete viral genome sequence of all three segments. Comparisons of the viral amino acid sequences among phenuiviruses indicated that the detected virus shared 46%-70% sequence identity with known members of the Kaisodi group in the genus Uukuvirus. Furthermore, phylogenetic analysis of the viral proteins showed that the virus formed a cluster with the Kaisodi group viruses, suggesting that this was a novel virus, which was designated "Toyo virus" (TOYOV). Further investigation of TOYOV is needed, and it will contribute to understanding the natural history and the etiological importance of the Kaisodi group viruses.