miR-214-5p Targets ROCK1 and Suppresses Proliferation and Invasion of Human Osteosarcoma Cells.
miR-214-5p Targets ROCK1 and Suppresses Proliferation and Invasion of Human Osteosarcoma Cells.
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DOI：
10.3727/096504016x14719078133401
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发表时间：
2017-01-02
影响因子：
3.1
通讯作者：
Yin R
中科院分区：
文献类型：
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作者：
Zhang M;Wang D;Zhu T;Yin R
microRNA（miRNAs）是一类在转录后水平调控特定靶基因的小分子保守RNA。它们参与了肿瘤进展的多个过程，包括细胞增殖。miR-214- 5 p（也称为miR-214*）是一种新发现的miRNA，其功能在很大程度上是未知的。本研究探讨miR-214- 5 p在人骨肉瘤（OS）细胞增殖和侵袭中的作用。结果显示，与正常组织和正常细胞相比，miR-214- 5 p在OS组织和细胞系中的表达明显降低。此外，miR-214- 5 p模拟物极大地提高了miR-214- 5 p水平，并显著降低了HOS和G293 OS细胞的增殖和侵袭。相反，miR-214- 5 p抑制剂对miR-214- 5 p水平、细胞增殖和细胞侵袭具有完全相反的作用。此外，生物信息学和荧光素酶报告基因分析证实，miR-1908靶向于ROCK 1 mRNA的3′-UTR区域，是一种新的肿瘤抑制因子，可直接靶向于ROCK 1，抑制人OS细胞的增殖。
MicroRNAs (miRNAs) are small conserved RNAs regulating specific target genes in posttranscriptional levels. They have been involved in multiple processes of tumor progression, including cell proliferation. miR-214-5p (also miR-214*) is a newly identified miRNA, and its functions are largely unknown. In this study, we explore the role of miR-214-5p in the proliferation and invasion of human osteosarcoma (OS) cells. The results showed that miR-214-5p was sharply reduced in OS tissues and cell lines, compared with normal tissues and cell lines. In addition, the miR-214-5p mimic greatly increased the miR-214-5p level and significantly decreased the proliferation and invasion of HOS and G293 OS cells. In contrast, the miR-214-5p inhibitor had a completely opposite effect on the miR-214-5p level, cell proliferation, and cell invasion. Moreover, bioinformatics and luciferase reporter gene assays confirmed that miR-1908 targeted the mRNA 3′-UTR region of ROCK1, a characterized tumor promoter in OS. In conclusion, miR-214-5p was identified as a new tumor suppressor, which directly targeted ROCK1 and suppressed proliferation of human OS cells.