Monitoring secretion in real time: Capacitance, amperometry and fluorescence compared
Monitoring secretion in real time: Capacitance, amperometry and fluorescence compared
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DOI：
10.1016/s0166-2236(97)01083-7
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发表时间：
1997-07-01
影响因子：
15.9
通讯作者：
Betz, WJ
中科院分区：
文献类型：
--
作者：
Angleson, JK;Betz, WJ
在活细胞中真实的时间测量胞吐、胞吞和囊泡循环的技术已经导致对神经元和其他分泌细胞中这些过程的知识的快速扩展。在过去十年中开发的几种实验方法在这种扩展中发挥了关键作用。在这篇综述中，我们专注于三种技术：电生理方法监测膜电容，电化学方法检测释放的分泌内容物和光学方法成像膜的内体和回收的囊泡，用荧光染料染色。每种技术都贡献了独特的和互补的信息囊泡周期，推进我们的知识膜融合和检索的动力学，分泌内容物的身份和空间模式和定向途径参与分泌膜回收。当然，每种技术都有其固有的局限性;最近，通过同时使用一种以上的方法，这些缺点中的一些已经得到解决。
Techniques for measuring exocytosis, endocytosis and vesicle cycling in living cells in real time have resulted in a rapid expansion in the knowledge of these processes in neurons and other secretory cells. Several experimental approaches, developed during the past decade, have played key roles in this expansion. In this review we focus on three techniques: electrophysiological methods for monitoring membrane capacitance, electrochemical methods for detecting released secretory contents and optical methods for imaging membranes of endosomes and recycled vesicles that are stained with fluorescent dyes. Each technique has contributed unique and complementary information about the vesicle cycle, advancing our knowledge of the kinetics of membrane fusion and retrieval, the identity of the secretory contents and the spatial patterns and directional pathways involved in secretory membrane recycling. Naturally, each technique has inherent limitations; some of these shortcomings have recently been resolved by using more than one method simultaneously.