DIRECT HAPLOTYPE DETERMINATION BY DOUBLE ARMS - SPECIFICITY, SENSITIVITY AND GENETIC APPLICATIONS
DIRECT HAPLOTYPE DETERMINATION BY DOUBLE ARMS - SPECIFICITY, SENSITIVITY AND GENETIC APPLICATIONS
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DOI：
10.1093/nar/19.13.3561
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发表时间：
1991-07-11
影响因子：
14.9
通讯作者：
WAINSCOAT, JS
中科院分区：
文献类型：
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作者：
LO, YMD;PATEL, P;WAINSCOAT, JS
我们已经开发了一种新的双扩增难治性突变系统（双ARMS）使用高度多态性区域5'到人类δ-珠蛋白基因作为模型系统。 双ARMS方法涉及在通过聚合酶链反应（PCR）进行DNA扩增期间同时使用两个等位基因特异性ARMS引物。 所得到的系统是高度敏感和更具体的比单一的ARMS。 此外，这种方法能够阐明同一染色体上多态性位点的关系，从而允许直接确定单倍型。 我们还证明了该系统可以与反向PCR结合使用，所得到的双ARMS反向PCR（DARMSI-PCR）可以允许在多态性位点上进行单倍型确定，所述多态性位点比PCR施加的长度限制分离得更远。 双ARMS方法在分子生物学中有许多其他应用，包括HLA分型、病毒学、法医病理学和骨髓移植后嵌合现象的研究。
We have developed a novel double Amplification Refractory Mutation System (double ARMS) using a highly polymorphic region 5' to the human delta-globin gene as a model system. The double ARMS approach involves using two allele-specific ARMS primers simultaneously during DNA amplification by the polymerase chain reaction (PCR). The resulting system is highly sensitive and more specific than single ARMS. In addition, this approach enables the elucidation of the relationship of polymorphic sites on the same chromosome and thus allows the direct determination of haplotypes. We have also demonstrated that this system can be used in conjunction with inverse PCR, the resulting double ARMS inverse PCR (DARMSI-PCR) may allow haplotype determination on polymorphic sites which are separated further apart than the length limit imposed by PCR. The double ARMS approach has numerous other applications in molecular biology including HLA typing, virology, forensic pathology and the investigation of the phenomenon of chimerism following bone marrow transplantation.