Effects of larval rearing temperature on immature development and West Nile virus vector competence of Culex tarsalis
Effects of larval rearing temperature on immature development and West Nile virus vector competence of Culex tarsalis
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DOI：
10.1186/1756-3305-5-199
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发表时间：
2012-09-11
影响因子：
3.2
通讯作者：
Rasgon, Jason L.
中科院分区：
文献类型：
--
作者：
Dodson, Brittany L.;Kramer, Laura D.;Rasgon, Jason L.
背景：众所周知，温度会引起蚊子生理、发育、生态的变化，并且在某些物种中会引起虫媒病毒载体能力的变化。由于定植蚊子在实验室条件下饲养，可能与现场的蚊子显着不同，因此实验室媒介能力实验可能无法准确反映自然媒介与病毒的相互作用。方法：我们评估了幼虫饲养温度对两种西尼罗河病毒（WNV）跗库蚊品系的未成熟发育参数和媒介能力的影响。 蚊子的发育参数，包括随着温度升高，化蛹和羽化时间缩短以及雌性体型变小。然而，感染性喂养后 5、7 和 14 天的西尼罗河病毒的感染、传播和传播率并没有受到一致的影响。结论：这些结果表明，不同的恒定幼虫饲养温度不会显着影响跗库蚊中西尼罗河病毒媒介能力的实验室估计。
Background: Temperature is known to induce changes in mosquito physiology, development, ecology, and in some species, vector competence for arboviruses. Since colonized mosquitoes are reared under laboratory conditions that can be significantly different from their field counterparts, laboratory vector competence experiments may not accurately reflect natural vector-virus interactions.Methods: We evaluated the effects of larval rearing temperature on immature development parameters and vector competence of two Culex tarsalis strains for West Nile virus (WNV).Results: Rearing temperature had a significant effect on mosquito developmental parameters, including shorter time to pupation and emergence and smaller female body size as temperature increased. However, infection, dissemination, and transmission rates for WNV at 5, 7, and 14 days post infectious feeding were not consistently affected.Conclusions: These results suggest that varying constant larval rearing temperature does not significantly affect laboratory estimates of vector competence for WNV in Culex tarsalis mosquitoes.