The reverse-direction method links mass experimental data to human diseases.
The reverse-direction method links mass experimental data to human diseases.
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反向方法将大量实验数据与人类疾病联系起来。
DOI：
10.1007/s00005-013-0255-9
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发表时间：
2014
影响因子：
3.2
通讯作者：
M
中科院分区：
文献类型：
--
作者：
Ogura;H.;Atsumi;T.;Bando;H.;Sabharwal;L.;Yamada;M.;Jiang;J. J.;Nakamura;A.;Arima;Y.;Kamimura;D. & Murakami;M
全基因组分析，如DNA微阵列，RNA测序和RNA干扰为基础的高通量筛选是普遍的破译感兴趣的生物过程，并提供大量的数据进行处理。研究人员的最终目标必须是将他们的数据外推到人类疾病。我们进行了功能性全基因组筛选，以阐明炎症放大器的分子机制，这是一种NFκB/STAT 3依赖性机制，可有效驱动免疫细胞的募集以促进炎症。利用全基因组关联研究（GWAS）的公共数据库，我们最近报道了反向方法，通过该方法，我们的大规模筛查数据成功地与许多人类疾病联系起来。例如，表皮调节素-表皮生长因子受体途径被GWAS确定为炎症放大器的调节因子，并与人类疾病相关。事实上，慢性炎症性疾病患者的血清表皮调节素水平更高。反向方法可以是一种有用的工具，可以缩小大量数据的范围，专注于人类疾病相关基因。
Genome-wide analyses such as DNA microarray, RNA sequencing and RNA interference-based high-throughput screening are prevalent to decipher a biological process of interest, and provide a large quantity of data to be processed. An ultimate goal for researchers must be extrapolation of their data to human diseases. We have conducted functional genome-wide screenings to elucidate molecular mechanisms of the inflammation amplifier, a NFκB/STAT3-dependent machinery that potently drives recruitment of immune cells to promote inflammation. Using a public database of genome-wide association studies (GWAS), we recently reported the reverse-direction method by which our mass screening data were successfully linked to many human diseases. As an example, the epiregulin–epidermal growth factor receptor pathway was identified as a regulator of the inflammation amplifier, and associated with human diseases by GWAS. In fact, serum epiregulin levels were higher in patients with chronic inflammatory disorders. The reverse-direction method can be a useful tool to narrow mass data down to focus on human disease-related genes.