MicroRNA-1 facilitates skeletal myogenic differentiation without affecting osteoblastic and adipogenic differentiation
MicroRNA-1 facilitates skeletal myogenic differentiation without affecting osteoblastic and adipogenic differentiation
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DOI：
10.1016/j.bbrc.2006.09.153
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发表时间：
2006-12-01
影响因子：
3.1
通讯作者：
Oh, Hidemasa
中科院分区：
文献类型：
--
作者：
Nakajima, Norio;Takahashi, Tomosaburo;Oh, Hidemasa
MicroRNA (miRNA) 是一种小型非编码 RNA，正在成为重要的转录后基因调控因子。在这项研究中，我们研究了 miR-1（一种在心肌和骨骼肌组织中特异性表达的 miRNA）对 C2Cl2 细胞的肌源性、成骨性和脂肪性分化的作用。在诱导肌原性分化后，miR-1 被强烈表达。逆转录病毒介导的 miR-1 过度表达显着增强了肌肉肌酸激酶、肌节肌球蛋白和 α-肌动蛋白的表达，而对肌细胞生成素和 MyoD 表达的影响不大。在 miR-1 过表达的细胞中，肌管的形成显着增强，表明 miR-1 的表达不仅增强了肌原性分化，而且还增强了肌管的成熟。相反，成骨细胞和脂肪形成分化不受 miR-1 强制表达的影响。因此，肌肉特异性 miRNA，miR-1，在控制谱系定型细胞的肌源性分化和成熟中发挥重要作用，而不是在命运决定中发挥作用。 (c) 2006 Elsevier Inc. 保留所有权利。
MicroRNAs (miRNAs) are small non-coding RNAs emerging as important post-transcriptional gene regulators. In this study, we examined the role of miR-1, an miRNA specifically expressed in cardiac and skeletal muscle tissue, on the myogenic, osteoblastic, and adipogenic differentiation of C2Cl2 cells. Upon induction of myogenic differentiation, miR-1 was robustly expressed. Retrovirus-mediated overexpression of miR-1 markedly enhanced expression of muscle creatine kinase, sarcomeric myosin, and alpha-actinin, while the effects on myogenin and MyoD expression were modest. Formation of myotubes was significantly augmented in miR-1-overexpressing cells, indicating miR-1 expression enhanced not only myogenic differentiation but also maturation into myotubes. In contrast, osteoblastic and adipogenic differentiation was not affected by forced expression of miR-1. Thus, the muscle-specific miRNA, miR-1, plays important roles in controlling myogenic differentiation and maturation in lineage-committed cells, rather than functioning in fate determination. (c) 2006 Elsevier Inc. All rights reserved.