Olfaction: Transient expression of a putative odorant receptor in the avian notochord
Olfaction: Transient expression of a putative odorant receptor in the avian notochord
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DOI：
10.1073/pnas.94.9.4766
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发表时间：
1997-04-29
影响因子：
11.1
通讯作者：
Nef, P
中科院分区：
文献类型：
--
作者：
Nef, S;Nef, P
在脊椎动物中，气味是由一个多基因家族控制的，该家族编码数百个在鱼类、大鼠、驼鹿、狗和人类中发现的7个跨膜结构域的G蛋白偶联受体。本研究进一步鉴定了鸡嗅觉受体亚家族7（COR7），该家族由两个高度相关的基因（COR7a和COR7b）组成，同源性为98.5%。通过原位杂交实验，在鸡胚胎das 6（E6）至新生期的嗅上皮中检测到COR7a和COR7b的转录本。在嗅上皮内，COR7a和COR7b标记细胞的空间分布是随机的，我们还观察到每个阳性细胞都不同时表达COR7a和COR7b基因。有趣的是，发现COR 7b基因在脊索中从E2到E6瞬时表达，而COR 7a或COR基因家族的任何其他已知成员在该中胚层组织中未检测到。这些数据表明，除了其在嗅觉系统中作为OR的潜在作用之外，COR7b也可能在脊索中具有对背腹组织或神经管和体细胞中胚层至关重要的功能。我们还讨论了可能的作用（S）的一个假定的或目前在脊索和感觉嗅觉上皮。
In vertebrates, odors are thought to be detected by a multigene family encoding several hundreds of seven-transmembrane-domain G-protein-coupled receptors found in fish, rat, moose, dog, and human, Recently, the putative odorant receptor (OR) gene family in the chicken has been characterized, Twelve members have been isolated and subdivided irate six subfamilies. Herein, we have further characterized the chicken olfactory receptor subfamily 7 (COR7) composed of two highly related genes (named COR7a and COR7b) which are 98.5% identical, By in situ hybridization experiments, both COR7a and COR7b transcripts were detected in the olfactory epithelium from embryonic das 6 (E6) to the new born stage. Within the olfactory epithelium, the spatial distribution of COR7a and COR7b labeled cells was random, We also observed that every individual positive cell did not coexpress the COR7a and COR7b genes. Interestingly, the COR7b gene was found to be transiently expressed ire the notochord from E2 to E6, whereas COR7a or any of the other known members of the COR gene family were not detected in this mesodermal tissue, These data suggest that, in addition to its potential role as an OR in the olfactory system, COR7b may also hare a function in the notochord that is essential for the dorsoventral organization or the neural tube and of the somitic mesoderm. We also discuss the possible role(s) of a putative OR present in both the notochord and the sensory olfactory epithelium.