Engineering collagen fiber templates with oriented nanoarchitecture and concerns on osteoblast behaviors
Engineering collagen fiber templates with oriented nanoarchitecture and concerns on osteoblast behaviors
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DOI：
10.1016/j.ijbiomac.2021.06.072
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发表时间：
2021-06-19
影响因子：
8.2
通讯作者：
Li, Hong
中科院分区：
文献类型：
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作者：
Luo, Xueshi;Zhang, Shuyun;Li, Hong
纳米结构为细胞提供了更接近天然骨结构的结构支持，并进一步调节细胞的行为，从而形成功能组织。在这项工作中，通过静电纺丝（Es），塑性压缩和拉伸（PCT）和动态剪切应力（SS）方法制备了三个具有定向纤维结构的工程胶原模板。POM、SEM、AFM和TEM观察发现，PCT-template和SS-template均包裹了具有67 nm d周期性的天然胶原结构的定向良好的纳米纤维，其力学性能优于Estemplate。提到细胞的行为，MC3T3-E1在pct -模板和ss -模板上培养时，均坚持沿胶原纤维取向的方向生长。具有纳米和微有序结构的ss模板引导细胞沿胶原纤维取向拉伸血浆，产生更多排列整齐的I型胶原纤维，促进MC3T3-E1的成骨分化明显高于pct -模板和es -模板。总之，我们强烈地争论分层胶原纤维结构用于骨组织再生的可行性。
Nanostructure provides a closer structural support approximation to native bone architecture for cells and further regulates cell's behavior, resulting in the formation of functional tissues. In this work, three engineering collagen templates with oriented fiber architectures were fabricated via electrospinning (Es), plastic compression and tensile (PCT), and dynamic shear stress (SS) methods. Under the observation of POM, SEM, AFM and TEM, the PCT-template and SS-template are packed with well-oriented nanofibers with the native collagen architecture of 67 nm D-periodicity, and the mechanical properties conferred to the templates are better than that of the Estemplate. When mentioning the cell's behavior, MC3T3-E1 adhered to grow along the alignment of collagen fiber orientation when cultured on the PCT-template and SS-template. The SS-template with nano- and micro-ordered architecture guided cells to stretch their plasma along with the orientation of collagen fiber, produce more aligned Type I collagen fibers and promote significantly higher osteogenic differentiation of MC3T3-E1 than the PCT-template and Es-template. Overall, it is strongly argued the feasibility of hierarchical collagen fiber architectures for bone tissue regeneration.