Cytocompatibility of the ready-to-use bioceramic putty repair cement iRoot BP Plus with primary human osteoblasts
Cytocompatibility of the ready-to-use bioceramic putty repair cement iRoot BP Plus with primary human osteoblasts
复制标题
DOI：
10.1111/j.1365-2591.2011.02003.x
复制
发表时间：
2012-06-01
影响因子：
5
通讯作者：
Granjeiro, J. M.
中科院分区：
文献类型：
--
作者：
De-Deus, G.;Canabarro, A.;Granjeiro, J. M.
De-Deus G，Canabarro A，Alves GG，Marins Jr，Linhares ABR，Granjeiro JM。即用型生物陶瓷油灰修复水泥iRoot BP Plus与原代人成骨细胞的细胞相容性《国际牙髓杂志》，45,508513,2012年。摘要目的验证iRoot BP Plus(IRoot BP Plus)的体外细胞相容性，并与White ProRoot MTA(MTA)进行比较。方法采用后退张开法预备36个人上前牙根管。垂直于根管长轴切开根尖3 mm，用超声仪加金刚石后刀头连续用水冲洗，制备根端空洞，制作标准化制剂。然后，用iRoot或MTA填充根端腔，每根暴露于细胞培养液中24或48h，将所获得的提取物暴露于人成骨细胞，进行多参数细胞存活率测定，评估线粒体活性、膜完整性和细胞密度。结果采用单因素方差分析，并辅以邓肯后验(P<；0.05)。结果在两个实验时间段，MTA处理的细胞均表现出与未处理细胞(阴性对照)相似的细胞相容性模式(P>；0.05)。然而，iRoot在48小时后促进的存活率明显低于MTA和对照组(P<；0.001)。然而，iRoot没有引起严重的细胞毒性效应，因为在大多数测试中，细胞存活率仍然高于对照组的70%。结论iRoot和MTA具有良好的生物相容性，不会引起严重的细胞毒作用。
De-Deus G, Canabarro A, Alves GG, Marins JR, Linhares ABR, Granjeiro JM. Cytocompatibility of the ready-to-use bioceramic putty repair cement iRoot BP Plus with primary human osteoblasts. International Endodontic Journal, 45, 508513, 2012. Abstract Aim To verify the in vitro cytocompatibility of iRoot BP Plus (iRoot) and to compare it with White ProRoot MTA (MTA). Methodology Thirty-six human maxillary incisor root canals were prepared using a step-back flaring technique. The apical 3 mm was resected perpendicular to the long axis at the roots, and root-end cavities were prepared with the aid of an ultrasonic device plus a diamond retrotip with continuous irrigation using water, producing standardized preparations. After that, the root-end cavities were filled with iRoot or MTA, and each root was exposed to cell culture media for 24 or 48 h. Human osteoblast cells were exposed to the extracts thus obtained, and a multiparametric cell viability assay was performed, evaluating mitochondrial activity, membrane integrity and cell density. The results were analysed by one-way analysis of variance, complemented with the Duncan post-test (P < 0.05). Results Cells exposed to MTA revealed a cytocompatibility pattern similar to the untreated cells (negative control), at both experimental times (P > 0.05). iRoot, however, promoted a significantly poorer viability than MTA and the control, after 48 h of exposure (P < 0.001). Nevertheless, iRoot did not induce critical cytotoxic effects because cell viability remained higher than 70% of the control group in most tests performed. Conclusion iRoot and MTA were biocompatible and did not induce critical cytotoxic effects.