ADAMTS-like 2 (ADAMTSL2) is a secreted glycoprotein that is widely expressed during mouse embryogenesis and is regulated during skeletal myogenesis
ADAMTS-like 2 (ADAMTSL2) is a secreted glycoprotein that is widely expressed during mouse embryogenesis and is regulated during skeletal myogenesis
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DOI：
10.1016/j.matbio.2007.03.003
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发表时间：
2007-07-01
期刊：
影响因子：
6.9
通讯作者：
Apte, Suneel S.
中科院分区：
文献类型：
--
作者：
Koo, Bon-Hun;Le Goff, Carine;Apte, Suneel S.
ADAMTS样2（ADAMTSL 2）是一种分泌蛋白，类似于ADAMTS蛋白酶的辅助结构域，但具有独特的结构特征。它有7个1型血小板反应蛋白重复序列（TSRs），但有一个异常长的间隔子模块，在人类和小鼠中，它含有一个带有6个N-糖基化位点的新插入。在HEK 293 F和COS-I细胞中表达的ADAMTSL 2蛋白是细胞表面和细胞外基质结合糖蛋白，其中N-连接的碳水化合物构成类似于20质量%。北方印迹法发现4.0 kb的Adamtsl 2 mRNA在成年小鼠的肝、肺和脾中含量最高。在小鼠胚胎发生过程中，Adamtsl 2在妊娠第三周表达最强。原位杂交检测Adamts 1 2 mRNA在发育中的骨骼肌、肝脏、支气管和动脉平滑肌、皮肤、椎间盘、软骨膜、胰腺和脊髓中的表达。ADAMTSL 2蛋白的免疫组织化学定位与mRNA表达相似。在发育中的骨骼肌管中检测到Adamtsl 2 mRNA和蛋白，但未分化的成肌前体导致我们研究其在体外成肌分化过程中的调节。在C2 C12和23 A2肌源性细胞中，但在通过表达G12 V：H-Ras（9A 2细胞）而呈现非肌源性的23 A2细胞中，由血清饥饿诱导的分化触发了Adamts 12 mRNA的表达，与肌肉分化的标志物Myog协调。此外，10 T1/2成纤维细胞中关键的肌原性决定簇MyoD的激活也触发了Adamtsl 2 mRNA的表达。总的来说，这些数据表明，Adamtsl 2 mRNA的诱导是肌发生的一个组成部分。(c)2007年Elsevier B. V./国际矩阵生物学学会。All rights reserved.
ADAMTS-like 2 (ADAMTSL2), is a secreted protein resembling the ancillary domains of the ADAMTS proteases, but with distinct structural features. It has 7 thrombospondin type-1 repeats (TSRs), but an unusually long spacer module, which in both humans and mice, contains a novel insertion bearing six N-glycosylation sites. The ADAMTSL2 protein expressed in HEK293F and COS-I cells, is a cell-surface and extracellular matrix binding glycoprotein, with N-linked carbohydrate constituting similar to 20% by mass. The 4.0 kb Adamtsl2 mRNA is found most abundantly in adult mouse liver, lung and spleen by northern blotting. During mouse embryogenesis, Adamtsl2 was expressed most strongly in the third week of gestation. Adamtsl2 mRNA was detected by in situ hybridization in developing skeletal muscle, liver, bronchial and arterial smooth muscle, skin, intervertebral disc, perichondrium, pancreas and spinal cord. Immunohistochemical localization of ADAMTSL2 protein was similar to mRNA expression. Detection of Adamtsl2 mRNA and protein in developing skeletal myotubes, but not undifferentiated myogenic precursors led us to investigate its regulation during in vitro myogenic differentiation. In C2C12 and 23A2 myogenic cells, but not in 23A2 cells rendered non-myogenic by expression of G12V:H-Ras (9A2 cells), differentiation induced by serum starvation triggered expression of Adamtsl2 mRNA, coordinately with Myog, a marker of muscle differentiation. Furthermore, activation of the key myogenic determinant MyoD in 10T1/2 fibroblasts also triggered expression of Adamtsl2 mRNA. Collectively, the data suggest that induction of Adamtsl2 mRNA is an integral feature of myogenesis. (c) 2007 Elsevier B.V./International Society of Matrix Biology. All rights reserved.