A novel type A2 neurotoxin gene cluster in Clostridium botulinum strain Mascarpone
A novel type A2 neurotoxin gene cluster in Clostridium botulinum strain Mascarpone
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DOI：
10.1111/j.1574-6968.2006.00331.x
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发表时间：
2006-08-01
影响因子：
2.1
通讯作者：
Aureli, Paolo
中科院分区：
文献类型：
--
作者：
Franciosa, Giovanna;Maugliani, Antonella;Aureli, Paolo
确定了A型肉毒梭菌菌株Mascarbotulinum中编码肉毒杆菌神经毒素复合物的基因簇的部分核苷酸序列（类似于10 kb）。分析揭示了六个ORF（orfs），其分别组织为菌株Kyoto-F和NCTC 2916的A2型和A3型肉毒杆菌神经毒素基因簇。虽然在序列的近端和远端的orf（orfX 2和bont/A基因）与菌株Kyoto-F的相应序列具有高水平的相似性，但序列内包含orfX 1和botR/A基因的片段与菌株NCTC 2916中的相关区域表现出更高程度的同源性。马斯卡虫神经毒素基因簇的镶嵌结构表明重组交换。
The partial nucleotide sequence (similar to 10 kb) of the cluster of genes encoding the botulinum neurotoxin complex in Clostridium botulinum type A strain Mascarpone was determined. The analysis revealed six ORFs (orfs), which were organized as in the type A2 and type A3 botulinum neurotoxin gene clusters of strains Kyoto-F and NCTC 2916, respectively. While the orfs at the proximal and distal ends of the sequence (orfX2 and bont/A genes) shared a high level of similarity with the corresponding sequences of strain Kyoto-F, the segment encompassing the orfX1 and botR/A genes within the sequence exhibited a higher degree of homology to the related region in strain NCTC 2916. The mosaic structure of the Mascarpone neurotoxin gene cluster suggests recombinational exchanges.