Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells
Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells
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DOI：
10.1902/jop.2004.75.9.1281
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发表时间：
2004-09-01
影响因子：
4.3
通讯作者：
Kurihara, H
中科院分区：
文献类型：
--
作者：
Kawaguchi, H;Hirachi, A;Kurihara, H
背景资料：使用合适的细胞移植到牙周骨缺损似乎是一个强大的战略，以促进牙周组织再生。骨髓间充质干细胞（Mesenchymal stem cells，MSCs）具有多向分化潜能。本研究的目的是检查是否自体移植的骨髓间充质干细胞到牙周骨缺损将是有益的牙周组织regeneration.Methods：骨髓间充质干细胞从比格犬分离，并在体外扩增。将扩增的MSC与终浓度为2 × 10（6）、5 × 10（6）、1 × 10（7）或2 × 10（7）个细胞/ml的去端胶原（2% I型胶原）混合，并自体移植到实验性III类缺损中。单独的去端胶原作为对照植入到缺损中。结果：MSC-去端肽胶原组牙周组织缺损处可再生出牙骨质、牙周膜和牙槽骨。与MSC-端肽胶原组相比，在对照组中观察到较少的牙周组织再生。形态计量学分析显示，5 × 10（6）和2 × 10（7）cells/ml组的新牙骨质长度百分比以及2 × 10（7）cells/ml组的新骨面积百分比均显著高于对照组结论：自体骨髓间充质干细胞移植是牙周组织再生的一种新方法。
Background: The use of suitable cells transplanted into periodontal osseous defects appears to be a powerful strategy to promote periodontal tissue regeneration. Mesenchymal stem cells (MSCs) isolated from bone marrow have the potential for multilineage differentiation. The purpose of this study was to examine whether auto-transplantation of MSCs into periodontal osseous defects would be useful for periodontal tissue regeneration.Methods: Bone marrow MSCs were isolated from beagle dogs and expanded in vitro. The expanded MSCs were mixed with atelocollagen (2% type I collagen) at final concentrations of 2 x 10(6), 5 x 10(6), 1 x 10(7), or 2 x 10(7) cells/ml, and auto-transplanted into experimental Class III defects. Atelocollagen alone was implanted into the defects as a control. Periodontal tissue healing was evaluated by histological and morphometric analyses 1 month after transplantation.Results: The defects were regenerated with cementum, periodontal ligament, and alveolar bone in the MSC-atelocollagen groups. Less periodontal tissue regeneration was observed in the control group compared to the MSC-atelocollagen groups. Morphometric analysis revealed that the percentage of new cementum length in the 5 x 10(6) and 2 x 10(7) cells/ml groups and the percentage of new bone area in the 2 x 10(7) cells/ml group were significantly higher than in the control group (P < 0.01).Conclusion: These findings suggest that auto-transplantation of bone marrow mesenchymal stem cells is a novel option for periodontal tissue regeneration.