Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction
Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction
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DOI：
10.1016/j.virol.2009.11.035
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发表时间：
2010-02-20
期刊：
影响因子：
3.7
通讯作者：
Suzuki, Nobuhiro
中科院分区：
文献类型：
--
作者：
Eusebio-Cope, Ana;Sun, Liying;Suzuki, Nobuhiro
呼肠孤病毒科MyRV1的两个片段S6和S10的重排以前被证明是由一种不同的单链RNA病毒CHV1-EP713(Sun和Suzuki，RNA 14,2557-2571,2008)编码的多功能蛋白p29高度诱导的。在这里，我们报告了MyRV1 S4重排的发生。尽管频率很低，但在没有CHV1 p29的情况下，导致S4中开放阅读框架(ORF)的内部80%-90%的缺失。对野生型MyRV1及其携带S4、S4+S10和S10重排的变异体感染的真菌菌株的比较分析表明，S4编码的VP4与VP10一样，对病毒复制不是必需的，但对于MyRV1引起的有效垂直传播和症状表达是必需的。这是呼肠孤病毒变种的第一个例子，它在两个基因组片段中携带超过75%的ORF缺失，并且仍然具有复制能力。(C)2009 Elsevier Inc.保留所有权利。
Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557-2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4. albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80-90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 Plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent. (C) 2009 Elsevier Inc. All rights reserved.