Expression of BMP-2 and BMP-4 proteins by type-1 and type-2 astrocytes induced from neural stem cells under different differentiation conditions.
Expression of BMP-2 and BMP-4 proteins by type-1 and type-2 astrocytes induced from neural stem cells under different differentiation conditions.
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DOI：
10.55782/ane-2012-1884
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发表时间：
2012-03
影响因子：
1.4
通讯作者：
Jian-Guo Hu;Yu-Xin Zhang;Q. Qi;Rui Wang;Lin Shen;Chen Zhang;Jin Xi;Jian-sheng Zhou;He-zuo Lu
中科院分区：
文献类型：
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作者：
Jian-Guo Hu;Yu-Xin Zhang;Q. Qi;Rui Wang;Lin Shen;Chen Zhang;Jin Xi;Jian-sheng Zhou;He-zuo Lu
骨形态发生蛋白（BMPs）是TGF-β超家族的一个亚组，在神经前体细胞命运的决定中起着关键作用。神经干细胞（neural stem cells，NSCs）是一种多能祖细胞，在一定条件下可分化为神经元、少突胶质细胞和星形胶质细胞。在我们最近的报告中，使用可以识别BMP-2和BMP-4（BMP-2/4）的抗体，我们表明BMP-2/4仅在含有1%胎牛血清（FBS）的培养基中从NSC分化的星形胶质细胞中表达。在这个体外模型中，神经干细胞的星形胶质细胞分化主要是向2型。当神经干细胞在含10%FBS的培养基中培养时，大多数细胞分化为1型星形胶质细胞。然而，在这些不同的培养条件下，从NSC诱导的1型和2型星形胶质细胞中BMP-2和BMP-4表达的信息很少。本研究采用BMP-2和BMP-4特异性抗体，通过RT-PCR、Western blot和免疫荧光染色，分别检测了1%和10%FBS培养条件下神经干细胞及其衍生物中BMP-2和BMP-4的表达。我们发现BMP-2和BMP-4在1型和2型星形胶质细胞中高度表达，而在神经干细胞、神经元和少突胶质细胞中没有检测到表达。提示星形胶质细胞可能是神经干细胞分化过程中骨形成蛋白的来源之一。然而，在我们的模型中，我们不能排除小胶质细胞或内皮细胞也可能是BMP来源的可能性。
Bone morphogenetic proteins (BMPs), a subgroup of the TGF-β superfamily, play critical roles in neural progenitor cell fate determination. Neural stem cells (NSCs) are multipotent progenitor cells that can differentiate into neurons, oligodendrocytes and astrocytes under certain conditions. In our recent report, using an antibody that can recognize both BMP-2 and BMP-4 (BMP-2/4), we showed that BMP-2/4 is only expressed in astrocytes differentiated from NSCs in a medium containing 1% fetal bovine serum (FBS). In this in vitro model, the astrocytic differentiation of NSCs was mainly toward type-2. When NSCs were cultured in a medium containing 10% FBS, most of the cells differentiated into type-1 astrocytes. However, little information is available for BMP-2 and BMP-4 expression in type-1 and type-2 astrocytes induced from NSCs under these different culture conditions. In this study, using two antibodies specific for BMP-2 and BMP-4, respectively, we discriminated the presence of BMP-2 and BMP-4 in NSCs and their derivatives under 1% and 10% FBS culture conditions by RT-PCR, western blot and immunofluorescence staining. We found that BMP-2 and BMP-4 are highly expressed in both type-1 and type-2 astrocytes, and no detectable expression in NSCs, neurons and oligodendrocytes. This suggests that the astrocytes might be one source of BMPs during the differentiation of NSCs. However, in our model, we cannot exclude the possibility that microglia or endothelial cells could also be a source of BMPs.