INTERLEUKIN‐2 PRODUCTION IN THE NEONATAL MOUSE
INTERLEUKIN‐2 PRODUCTION IN THE NEONATAL MOUSE
复制标题
新生小鼠中白细胞介素-2 的产生
作者：
B. Argyris;M. DeStefano;K. Zamkoff
新生小鼠的脾和胸腺细胞在体外不能产生IL-2。在体外，脾细胞产生的IL-2在20日龄至27日龄之间开始发育，并在40日龄时达到成年水平。胸腺细胞在体外不具备产生IL-2的能力。在成人脾细胞培养中加入新生儿脾、新生儿胸腺或成人胸腺细胞会抑制IL-2的产生。用丝裂霉素处理添加的细胞并不能消除它们的抑制活性。从新生儿脾、新生儿胸腺或成人胸腺的三天细胞培养中获得的可溶性因子也抑制成人脾细胞培养中IL-2的产生。在成人胸腺、新生儿脾或新生儿胸腺细胞培养中加入IL-2后，IL-2的产生略有增加，但在没有IL-1的情况下，IL-2的产生仍显著低于成人脾。结果表明，在新生儿的脾和胸腺中存在产生IL-2的抑制细胞。脾抑制细胞的活性在出生后消失，但胸腺抑制细胞的活性保留到成年。
Neonatal mouse spleen and thymus cells fail to produce interleukin-2 (IL-2) in vitro. In vitro IL-2 production by spleen cells begins to develop between 20 and 27 days of age and reaches adult levels at 40 days of age. Thymus cells do not develop the ability to produce IL-2 in vitro. Addition of neonatal spleen, neonatal thymus, or adult thymus cells to adult spleen cell cultures inhibits IL-2 production. Treatment of the added cells with mitomycin does not abrogate their suppressor activity. Soluble factors, obtained from three-day cell cultures of neonatal spleen, neonatal thymus, or adult thymus also suppress IL-2 production in adult spleen cell cultures. Addition of interleukin-raises IL-2 production in adult thymus, neonatal spleen, or neonatal thymus cell cultures slightly, but the IL-2 productivity is still considerably lower than that of adult spleen in the absence of IL-1. The results indicate that suppressor cells for IL-2 production are present in neonatal spleen and thymus. The splenic suppressor cell activity disappears after birth, but thymic suppressor cell activity is retained into adulthood.