CONFORMATIONAL STUDIES OF POLY-ALPHA-AMINO ACIDS WITH AROMATIC-SIDE-CHAIN EFFECTS
CONFORMATIONAL STUDIES OF POLY-ALPHA-AMINO ACIDS WITH AROMATIC-SIDE-CHAIN EFFECTS
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DOI：
10.1021/ar50009a003
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发表时间：
1968-01-01
影响因子：
18.3
通讯作者：
BENEDETT.E
中科院分区：
文献类型：
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作者：
GOODMAN, M;DAVIS, GW;BENEDETT.E
近年来，科学家们通过X射线衍射分析，在对纤维状和球状蛋白质结构的理解上取得了革命性的进展。鲍林的α螺旋，1佩鲁茨的2类质同象替换，以及科克伦、克里克和范德3推导螺旋X射线散射的表达式都是在这一时期建立的。对肌红蛋白、4血红蛋白、6溶菌酶、8和核糖核酸酶进行了完整的结构测定。7预期在完全生物活性的条件下，在底物存在下对这些蛋白质的X射线衍射分析将导致阐明这些生物大分子的结构-功能关系。8许多生物物质不能结晶，因此不能用X射线衍射分析进行研究。即使当生物聚合物可以结晶时，找到多个同晶重原子衍生物也是一个命中或失败的命题。许多工作者已经证明，多肽可以形成螺旋，并且它们保持
In recent years scientists have achieved a revolution in the understandingof the structure of fibrous and globular proteins by means of X-ray diffraction analysis. Pauling’s a helix, 1 Perutz’s2 isomorphous replacement, and the derivation of expressions of X-ray scattering by helices by Cochran, Crick, and Vand3 were all established during this period. Complete structural determinations havebeen presented for myoglobin, 4 hemoglobin, 6 lysozyme, 8 and ribonuclease. 7 It is expected that X-ray diffraction analysis of these proteins in the presence of substratesunder conditions of complete biological activity will lead toelucidation of structure-function relationships for these biological macromolecules. 8Many biological substances cannot be crystallized and thus cannot be investigated byX-ray diffraction analysis. Even when biopolymers can be crystallized, it is a hit or miss proposition to find multiple isomor-phous heavy atom derivatives. It has been demonstrated by many workers that polypeptides can form a helices and that they maintain