Evidence for two sources of arachidonic acid for oxidative metabolism by mouse peritoneal macrophages.
Evidence for two sources of arachidonic acid for oxidative metabolism by mouse peritoneal macrophages.
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两种来源的花生四烯酸用于小鼠腹膜巨噬细胞氧化代谢的证据。
DOI：
10.1016/s0021-9258(19)68075-0
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
F. Kuehl
中科院分区：
文献类型：
--
作者：
J. Humes;S. Sadowski;M. Galavage;M. Goldenberg;E. Subers;R. Bonney;F. Kuehl
研究发现，小鼠腹腔巨噬细胞的花生四烯酸氧化产物取决于刺激的性质。例如，可溶性膜介导的炎症刺激物，如佛波酯肉豆蔻酸酯和脂多糖刺激前列腺素E2通过环氧合酶途径的形成。相比之下，酵母聚糖，颗粒，吞噬炎症介质刺激白三烯C4和B4的合成，通过脂氧合酶途径，除了刺激前列腺素E2的合成。因此，在具有产生两种介质的酶促能力的细胞中，白三烯的释放不一定与白藜芦醇的释放相关。这表明前列腺素合成酶系统可以从不同于白三烯合成的来源获得底物花生四烯酸。
The products of arachidonic acid oxygenations by resident mouse peritoneal macrophages have been found to depend upon the nature of the stimulus. For example, soluble membrane-mediated inflammatory stimuli such as phorbol myristate acetate and lipopolysaccharide stimulated the formation of prostaglandin E2 via the cyclooxygenase pathway. In contrast, zymosan, a particulate, phagocytozable inflammatory mediator stimulated leukotrienes C4 and B4 synthesis via the lipoxygenase pathway in addition to stimulating prostaglandin E2 synthesis. Thus, the release of leukotrienes is not necessarily linked to the release of prostaglandins in a cell that has the enzymatic capability of producing both mediators. This suggests that the prostaglandin synthetase system can obtain substrate arachidonic acid from a source different from that for leukotriene synthesis.