Longitudinal study of daily variation of rats' behavior in the elevated plus-maze
Longitudinal study of daily variation of rats' behavior in the elevated plus-maze
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DOI：
10.1016/s0031-9384(02)00941-1
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发表时间：
2003-01-01
影响因子：
2.9
通讯作者：
de Andrade, TGCS
中科院分区：
文献类型：
--
作者：
Andrade, MMM;Tomé, MF;de Andrade, TGCS
本研究对Wistar雄性大鼠在出生后1、2、3、6、12、18个月高架十字迷宫（高架十字迷宫）中行为的日变化进行了纵向研究。将动物以2小时间隔分12个阶段进行十字迷宫（n=72，每个时间点6只）。通过观察24小时录像记录，评估了4只动物的自发性静息-活动节律。时间序列分析采用Cosinor方法。通过重复测量的双因素方差分析比较了六种情况下以及在光照和黑暗阶段的行为率。在光照阶段和老年动物中，EPM的探索行为较小。在黑暗阶段的第一个月和第三个月，以及在光明阶段的第一个月，观察到更高的开放和闭合臂探索值。调整到24小时内是显着的，在所有阶段的休息活动数据，在封闭的武器，和中心的时间条目数，并为三至五个阶段的开放臂探索。一般而言，24小时变异性在年轻动物中比老年动物更明显。本研究表明：（1）所分析的行为指标的显著总变异性可归因于24小时的变异，（2）在所有发育阶段都存在着对大脑皮层探索的亮/暗时相差异，（3）与年轻大鼠相比，老年Wistar大鼠对大脑皮层的探索较少，（4）行为指数（BAI）的下降与时相有关，部分与静息-活动节律的重组有关。(C)2003年爱思唯尔科学公司All rights reserved.
We conducted a longitudinal study about daily variation of Wistar male rats' behavior in the elevated plus-maze (EPM) evaluated in the 1st, 2nd, 3rd, 6th, 12th, and 18th months of life. Animals were submitted to the plus-maze in 12 sessions at 2-h intervals (n=72, 6 per time point). Spontaneous rest-activity rhythm of four animals was assessed by observation of 24-h videotape records. Time series were analyzed by Cosinor method. Behavioral rates on the six occasions and in light and dark phases were compared by means of two-way ANOVA with repeated measures. Exploratory behavior in EPM was smaller in the light phase and in older animals. Higher values of open and closed arms exploration were observed in the first and third months of the dark phase, and in the first month of the light phase. Adjustment to the 24-h period was significant at all stages for rest-activity data, number of entries in closed arms, and time on center, and for three to five stages for open-arm exploration. In general, 24 h variability was more pronounced in younger animals compared with older ones. The present study showed that: (1) a significant amount of total variability of the behavioral indexes analyzed could be attributed to 24 h variation, (2) light/dark phases differences in EPM exploration were present at all developmental stages, (3) older Wistar rats explored less the EPM and were less active in their home cage compared with younger ones, and (4) behavioral indexes (EPM) decrease was phase related and partially related to a reorganization of rest-activity rhythm. (C) 2003 Elsevier Science Inc. All rights reserved.