Distribution of intranasal instillations in mice: effects of volume, time, body position, and anesthesia
Distribution of intranasal instillations in mice: effects of volume, time, body position, and anesthesia
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DOI：
10.1152/ajplung.00173.2001
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发表时间：
2002-04-01
影响因子：
4.9
通讯作者：
Inman, MD
中科院分区：
文献类型：
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作者：
Southam, DS;Dolovich, M;Inman, MD
鼻内滴注技术用于将各种物质递送到小鼠的上呼吸道和下呼吸道（URT和LRT）。在这里，我们量化的相对分布与鼻内递送的不可吸收的示踪剂，Tc-99 m标记的硫化物胶体。通过在滴注后处死小鼠并使用γ射线照相术定量解剖组织中的放射性来确定相对分布。当在气体麻醉下滴注后5分钟处死动物时，观察到输注量对相对分布的显著影响。当输送量为5穆尔时，在LRT中未检测到辐射;当输送50穆尔时，LRT中的辐射分布增加到最大值55.7 +/- 2.5%。在轻轨列车中未检测到的大部分辐射在城市轨道交通中发现。在随后的1小时内，LRT中的辐射保持恒定，而URT中的辐射减少并出现在胃肠道中。将25穆尔滴注到麻醉小鼠中导致30.1 +/-6.9%分布到LRT，而在清醒小鼠的LRT中仅检测到5.3 +/-1.5%（P < 0.05）的相同体积。改变小鼠的身体位置并不影响相对分布。当使用鼻内滴注时，URT和LRT与胃肠道之间的相对分布受到递送体积和麻醉水平的严重影响。
Intranasal instillation techniques are used to deliver various substances to the upper and lower respiratory tract (URT and LRT) in mice. Here, we quantify the relative distribution achieved with intranasal delivery of a nonabsorbable tracer, Tc-99m-labeled sulfide-colloid. Relative distribution was determined by killing mice after instillation and quantifying the radioactivity in dissected tissues using gamma scintigraphy. A significant effect of delivery volume on relative distribution was observed when animals were killed 5 min after instillation delivered under gas anesthesia. With a delivery volume of 5 mul, no radiation was detected in the LRT; this increased to a maximum of 55.7 +/- 2.5% distribution to the LRT when 50 mul were delivered. The majority of radiation not detected in the LRT was found in the URT. Over the course of the following 1 h, radiation in the LRT remained constant, while that in the URT decreased and appeared in the gastrointestinal tract. Instillation of 25 mul into anesthetized mice resulted in 30.1 +/- 6.9% distribution to the LRT, while only 5.3 +/- 1.5% (P < 0.05) of the same volume was detected in the LRT of awake mice. Varying the body position of mice did not affect relative distribution. When using intranasal instillation, the relative distribution between the URT and LRT and the gastrointestinal tract is heavily influenced by delivery volume and level of anesthesia.