ANALYSIS OF SPERMATOGENESIS IN DROSOPHILA-MELANOGASTER BEARING DELETIONS FOR Y-CHROMOSOME FERTILITY GENES
ANALYSIS OF SPERMATOGENESIS IN DROSOPHILA-MELANOGASTER BEARING DELETIONS FOR Y-CHROMOSOME FERTILITY GENES
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DOI：
10.1007/bf00328522
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发表时间：
1981-01-01
期刊：
影响因子：
1.6
通讯作者：
LINDSLEY, DL
中科院分区：
文献类型：
--
作者：
HARDY, RW;TOKUYASU, KT;LINDSLEY, DL
用电镜和光学显微镜检查了一系列连续的非重叠Y染色体缺陷对精子发生的影响。这些缺陷是通过组合不同X-Y易位的元件构建的;它们将Y细分为7个片段，其中6个片段是雄性育性所需的（长臂中4个，短臂中2个）。从初级精母细胞到成熟精子的形成，对精子发生进行了检查，并确定了最早偏离正常发育的精子。两个缺陷导致相同的结构从精子尾部的轴丝，动力蛋白的外臂延伸从A subtubule的外周双峰的情况下，他们也导致在初级精母细胞核的聚集体的微管在一种情况下，在其他网状物质的情况下。第三个缺陷导致初级精母细胞中出现X0雄性特有的晶体，以及精子细胞减数分裂期间核和细胞质元素的不规则分布。第四个缺陷导致发育中的轴丝与线粒体衍生物的错位，并且首先在精子细胞的洋葱nebenkern阶段检测到。对于2个缺陷，检测到的第一个异常发生在晚期，包括在大多数不育中发现的综合征。这种表型归因于早期病变的间接影响。
The effects on spermatogenesis of a series of contiguous nonoverlapping Y-chromosome deficiencies were examined using both EM and light microscopy. The deficiencies were constructed by combining elements of different X-Y translocation; they subdivide the Y into 7 segments, 6 of which are required for male fertility (4 in the long arm and 2 in the short arm). Spermatogenesis was examined from the primary spermatocyte through to the formation of mature sperm and the earliest departures from normal development identified. Two deficiencies result in the absence of the same structure from the axoneme of the sperm tail, the dynein-containing outer arm extending from the A subtubule of the peripheral doublet; they also result in the absence from primary spermatocyte nuclei of aggregates of tubuli in one case and reticular material in the other. A 3rd deficiency causes the appearance in the primary spermatocyte of the crystals characteristic of X0 males and the irregular distribution during meiosis of nuclear and cytoplasmic elements to the spermatids. The 4th deficiency results in the misalignment of the developing axoneme with the mitochondrial derivatives and is first detectable in the onion nebenkern stage of the spermatid. For 2 deficiencies the 1st abnormalities detected were during later stages and comprise a syndrome found in most of the steriles. This phenotype is attributed to the indirect effects of earlier lesions.