Backbone assignment of the binary complex of the full length Sulfolobus solfataricus DNA polymerase IV and DNA.
Backbone assignment of the binary complex of the full length Sulfolobus solfataricus DNA polymerase IV and DNA.
复制标题
全长硫磺硫化叶菌 DNA 聚合酶 IV 和 DNA 的二元复合物的主链分配。
DOI：
10.1007/s12104-016-9717-4
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发表时间：
2017
影响因子：
0.9
通讯作者：
Wu,Zhengrong
中科院分区：
文献类型：
--
作者：
Lee,Eunjeong;Fowler,JasonD;Suo,Zucai;Wu,Zhengrong
solfataricussulfolobus DNA聚合酶IV （Dpo4）是一种模型y家族DNA聚合酶，在体内和体外都能绕过多种DNA损伤。在本文中，我们报道了全长Dpo4在其与14/14-mer DNA底物的二元配合物中的主链化学位移分配。DNA结合后，分离的催化核心和Dpo4的小指/连接片段中的几个β-链区域变得更加结构化。这项工作为我们正在进行的Dpo4构象动力学研究和未来DNA聚合酶及其二元和三元配合物的第一溶液结构的确定奠定了基础。
Sulfolobus solfataricusDNA polymerase IV (Dpo4), a model Y-family DNA polymerase, bypasses a wide range of DNA lesions in vitro and in vivo. In this paper, we report the backbone chemical shift assignments of the full length Dpo4 in its binary complex with a 14/14-mer DNA substrate. Upon DNA binding, several β-stranded regions in the isolated catalytic core and little finger/linker fragments of Dpo4 become more structured. This work serves as a foundation for our ongoing investigation of conformational dynamics of Dpo4 and future determination of the first solution structures of a DNA polymerase and its binary and ternary complexes.