High-Casein Diet Suppresses Guanidinoacetic Acid-Induced Hyperhomocysteinemia and Potentiates the Hypohomocysteinemic Effect of Serine in Rats
High-Casein Diet Suppresses Guanidinoacetic Acid-Induced Hyperhomocysteinemia and Potentiates the Hypohomocysteinemic Effect of Serine in Rats
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DOI：
10.1271/bbb.80543
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发表时间：
2008-12-01
影响因子：
1.6
通讯作者：
Sugiyama, Kimio
中科院分区：
文献类型：
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作者：
Ohuchi, Seiya;Matsumoto, Yuko;Sugiyama, Kimio
为了确定膳食蛋白质水平对实验性高同型半胱氨酸血症的影响，给大鼠喂食 10% 酪蛋白 (10C) 和 40% 酪蛋白 (40C) 饮食，含或不含 0.5% 胍基乙酸 (GAA) 14 天。此外，给大鼠喂食10C + 0.75%蛋氨酸（10CM）和40C + 0.75%蛋氨酸（40CM）饮食，加或不加2.5%丝氨酸14天，以确定饮食蛋白质水平与丝氨酸低同型半胱氨酸作用强度之间的关系。 GAA 的补充显着增加了喂食 10C 饮食的大鼠血浆同型半胱氨酸浓度，而没有增加喂食 40C 饮食的大鼠血浆同型半胱氨酸浓度。虽然丝氨酸的补充显着抑制了蛋氨酸引起的血浆同型半胱氨酸浓度的升高，但喂食40CM饮食的大鼠血浆同型半胱氨酸浓度的降低明显低于喂食10CM饮食的大鼠。无论是否补充 GAA 和丝氨酸，饲喂 40C 或 40CM 饮食的大鼠的肝脏胱硫醚 β-合酶和甜菜碱-同型半胱氨酸 S-甲基转移酶活性均显着高于饲喂 10C 或 10CM 饮食的大鼠。这些结果表明，高酪蛋白饮食可以有效抑制 GAA 诱导的高同型半胱氨酸血症，并增强丝氨酸的低同型半胱氨酸血症作用，这可能是通过增强同型半胱氨酸代谢酶的活性来实现的。
To determine the effect of dietary protein level on experimental hyperhomocysteinemia, rats were fed 10% casein (10C) and 40% casein (40C) diets with or without 0.5% guanidinoacetic acid (GAA) for 14d. In addition, rats were fed 10C + 0.75% methionine (10CM) and 40C + 0.75% methionine (40CM) diets with or without 2.5% serine for 14d to determine the relationship between the dietary protein level and intensity of the hypohomocysteinemic effect of serine. GAA supplementation markedly increased the plasma homocysteine concentration in rats fed with the 10C diet, whereas it did not increase the plasma homocysteine concentration in rats fed with the 40C diet. Although serine supplementation significantly suppressed the methionine-induced enhancement of plasma homocysteine concentration, the decreased plasma homocysteine concentration was significantly lower in rats fed with the 40CM diet than in rats fed with the 10CM diet. The hepatic cystathionine beta-synthase and betaine-homocysteine S-methyltransferase activities were significantly higher in rats fed with the 40C or 40CM diet than in rats fed with the 10C or 10CM diet, irrespective of supplementation with GAA and serine. These results indicate that the high-casein diet was effective for both suppressing GAA-induced hyperhomocysteinemia and potentiating the hypohomocysteinemic effect of serine, probably through the enhanced activity of homocysteine-metabolizing enzymes.