THE CYTOPLASMIC DOMAIN OF THE T-CELL RECEPTOR ZETA-CHAIN IS SUFFICIENT TO COUPLE TO RECEPTOR-ASSOCIATED SIGNAL TRANSDUCTION PATHWAYS
THE CYTOPLASMIC DOMAIN OF THE T-CELL RECEPTOR ZETA-CHAIN IS SUFFICIENT TO COUPLE TO RECEPTOR-ASSOCIATED SIGNAL TRANSDUCTION PATHWAYS
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DOI：
10.1016/0092-8674(91)90314-o
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发表时间：
1991-03-08
期刊：
影响因子：
64.5
通讯作者：
WEISS, A
中科院分区：
文献类型：
--
作者：
IRVING, BA;WEISS, A
T细胞抗原受体（TCR）不变链（CD 3-γ、δ、β和ζ）的功能知之甚少。 证据表明，CD 3偶联受体配体结合细胞内信号传导事件。 为了研究CD 3-ζ链在TCR介导的信号转导中的作用，构建了将CD 8的细胞外和跨膜结构域连接至ζ链的胞质结构域的嵌合蛋白。 CD 8/ζ-嵌合体独立于TCR表达，并且能够转导信号，根据早期和晚期活化的标准，所述信号与由完整TCR产生的信号不可区分。 这些数据表明，CD 8/zeta可以在不存在CD 3-γ、δ和γ的情况下激活适当的信号转导途径，并表明CD 3-zeta的作用是将TCR偶联至细胞内信号转导机制。
The function of the T cell antigen receptor (TCR) invariant chains, CD3-gamma, delta, epsilon, and zeta, is poorly understood. Evidence suggests that CD3 couples receptor ligand binding to intracellular signaling events. To examine the role of the CD3-zeta-chain in TCR-mediated signal transduction, a chimeric protein linking the extracellular and transmembrane domains of CD8 to the cytoplasmic domain of the zeta-chain was constructed. The CD8/zeta-chimera is expressed independently of the TCR and is capable of transducing signals that, by criteria of early and late activation, are indistinguishable from those generated by the intact TCR. These data indicate that CD8/zeta can activate the appropriate signal transduction pathways in the absence of CD3-gamma, delta, and epsilon, and suggest that the role of CD3-zeta is to couple the TCR to intracellular signal transduction mechanisms.