Evaluating biomechanical properties of murine embryos using Brillouin microscopy and optical coherence tomography
Evaluating biomechanical properties of murine embryos using Brillouin microscopy and optical coherence tomography
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DOI：
10.1117/1.jbo.22.8.086013
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发表时间：
2017-08-01
影响因子：
3.5
通讯作者：
Scarcelli, Giuliano
中科院分区：
文献类型：
--
作者：
Raghunathan, Raksha;Zhang, Jitao;Scarcelli, Giuliano
胚胎发生受细胞微环境的机械特性的许多变化调节。因此，研究胚胎机械生理学可以提供更全面的胚胎发育视角，可能改善先天性异常的早期检测以及评估和开发治疗干预措施。许多方法和技术已被用于研究胚胎发生过程中的细胞生物力学特性。虽然这些技术中的一些是侵入性的或涉及使用外部代理，其他人在空间和时间分辨率方面受到损害。我们建议使用布里渊显微镜结合光学相干断层扫描（OCT）来测量刚度以及发育中的胚胎的结构变化。虽然布里渊显微镜评估胚胎不同器官之间的硬度变化，但OCT提供了必要的结构指导。(C)2017年，摄影光学仪器工程师协会（SPIE）
Embryogenesis is regulated by numerous changes in mechanical properties of the cellular micro-environment. Thus, studying embryonic mechanophysiology can provide a more thorough perspective of embryonic development, potentially improving early detection of congenital abnormalities as well as evaluating and developing therapeutic interventions. A number of methods and techniques have been used to study cellular biomechanical properties during embryogenesis. While some of these techniques are invasive or involve the use of external agents, others are compromised in terms of spatial and temporal resolutions. We propose the use of Brillouin microscopy in combination with optical coherence tomography (OCT) to measure stiffness as well as structural changes in a developing embryo. While Brillouin microscopy assesses the changes in stiffness among different organs of the embryo, OCT provides the necessary structural guidance. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)