β-Catenin-Dependent Control of Positional Information along the AP Body Axis in Planarians Involves a Teashirt Family Member
β-Catenin-Dependent Control of Positional Information along the AP Body Axis in Planarians Involves a Teashirt Family Member
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DOI：
10.1016/j.celrep.2014.12.018
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发表时间：
2015-01-13
期刊：
影响因子：
8.8
通讯作者：
Bartscherer, Kerstin
中科院分区：
文献类型：
--
作者：
Reuter, Hanna;Maerz, Martin;Bartscherer, Kerstin
Wnt/ β -连环蛋白信号调节后生动物的组织稳态和再生。在涡虫（具有高再生潜能的扁虫）中，wnt配体被认为是通过沿前后轴形成β -连环蛋白活性梯度来控制组织极性，但其下游机制尚不清楚。我们进行了基于RNA测序（RNA-seq）的筛选，并鉴定了数百个β -连环蛋白依赖转录本，其中一些在肌肉组织和干细胞中以分级方式表达。特别是，在涡虫和斑马鱼的再生过程中，以β -catenin依赖的方式诱导了t恤衫（tsh）同源物，RNAi导致了双头涡虫。引人注目的是，完整的涡虫耗尽了tsh诱导的前标记物，并慢慢地将尾巴转变为头部，让人想起β -连环蛋白RNAi表型。考虑到β -连环蛋白RNAi增强了尾部区域表达前决定因子的肌肉细胞的形成，我们的研究表明，该途径通过调节体内平衡和再生过程中分化细胞的身份来控制组织极性。
Wnt/beta-catenin signaling regulates tissue homeostasis and regeneration in metazoans. In planarians-flatworms with high regenerative potential-Wnt ligands are thought to control tissue polarity by shaping a beta-catenin activity gradient along the anterior-posterior axis, yet the downstream mechanisms are poorly understood. We performed an RNA sequencing (RNA-seq)-based screen and identified hundreds of beta-catenin-dependent transcripts, of which several were expressed in muscle tissue and stem cells in a graded fashion. In particular, a teashirt (tsh) ortholog was induced in a beta-catenin-dependent manner during regeneration in planarians and zebrafish, and RNAi resulted in two-headed planarians. Strikingly, intact planarians depleted of tsh induced anterior markers and slowly transformed their tail into a head, reminiscent of beta-catenin RNAi phenotypes. Given that beta-catenin RNAi enhanced the formation of muscle cells expressing anterior determinants in tail regions, our study suggests that this pathway controls tissue polarity through regulating the identity of differentiating cells during homeostasis and regeneration.