CHARACTERIZATION OF INFECTIOUS TRANSCRIPTS FROM A POTATO VIRUS-X CDNA CLONE
CHARACTERIZATION OF INFECTIOUS TRANSCRIPTS FROM A POTATO VIRUS-X CDNA CLONE
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DOI：
10.1016/0042-6822(90)90421-m
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发表时间：
1990-04-01
期刊：
影响因子：
3.7
通讯作者：
TUMER, NE
中科院分区：
文献类型：
--
作者：
HEMENWAY, C;WEISS, J;TUMER, NE
构建了马铃薯X病毒(PVX)全长cDNA克隆，并将其与噬菌体T7启动子在体外转录载体中融合。从该模板获得的转录本(PMON 8660)在接种到局部病斑寄主橙色藜时具有感染性。这些转录本的感染力约为正品PVX RNA的0.2%。从接种了这些转录本的植物中采集的病变含有病毒颗粒和病毒聚集体，电子显微镜证明，这些病毒颗粒和病毒聚集体通常在接种了正品PVX RNA的植物的病变中观察到。此外，从这些病斑中分离出的子代病毒在接种到新的局部病斑植物上时，与从真正的PVX RNA感染中分离的子代病毒具有同样的传染性。来自PVX基因克隆的感染性转录本将有助于分析PVX感染的分子方面。
A full-length cDNA clone of potato virus X (PVX) has been constructed and fused to the bacteriophage T7 promoter in an in vitro transcription vector. Transcripts derived from this template (pMON 8660) were infectious when inoculated onto the local lesion host, Chenopodium amaranticolor. The infectivity of these transcripts was approximately 0.2% that of authentic PVX RNA. Lesions sampled from plants inoculated with these transcripts contained virus particles and virus aggregates typically observed in lesions from plants inoculated with authentic PVX RNA, as evidenced by electron microscopy. In addition, progeny virus isolated from these lesions was as infectious as progeny virus from an authentic PVX RNA infection when inoculated onto new local lesion plants. Infectious transcripts derived from PVX cDNA clones will facilitate analysis of the molecular aspects of PVX infection.